C57BL/6NCya-Cwf19l2em1flox/Cya
Common Name:
Cwf19l2-flox
Product ID:
S-CKO-08621
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cwf19l2-flox
Strain ID
CKOCMP-244672-Cwf19l2-B6N-VA
Gene Name
Product ID
S-CKO-08621
Gene Alias
3230401L03Rik; 9030209E10
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cwf19l2em1flox/Cya mice (Catalog S-CKO-08621) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027027
NCBI RefSeq
NM_027545
Target Region
Exon 6
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Cwf19l2, without common aliases mentioned, is a gene with significant functions. It is involved in regulating alternative splicing of pre-mRNA, which is crucial for various biological processes. The study of Cwf19l2 can be facilitated by genetic models like gene knockout (KO) or conditional knockout (CKO) mouse models.
In germline conditional Cwf19l2 knockout mice, male sterility is observed, along with impaired spermatogenesis characterized by increased apoptosis and decreased differentiated spermatogonia and spermatocytes. Cwf19l2 interacts with spliceosome proteins for proper spliceosome assembly and stability. It directly binds and regulates the splicing of genes related to spermatogenesis (Znhit1, Btrc, and Fbxw7) and RNA splicing (Rbfox1, Celf1, and Rbm10), and can also indirectly amplify its splicing-regulation effect through modulating RBFOX1 [1]. Also, through integrated transcriptomic and proteomic Mendelian randomization, a positive association between Cwf19l2 and the risk of coronary artery calcification (CAC) was found [2]. Additionally, in breast cancer patients, germline single nucleotide polymorphisms (SNPs) in the gene Cwf19l2, which encodes a protein involved in the regulation of the cell cycle, were associated with deletion of 16q [3].
In conclusion, Cwf19l2 is essential for male fertility and spermatogenesis by regulating alternative splicing. The Cwf19l2 KO/CKO mouse models have revealed its role in male-specific reproductive processes. Moreover, research has also linked Cwf19l2 to diseases such as coronary artery calcification and breast cancer, highlighting its significance in multiple disease areas.
References:
1. Wang, Shiyu, Cai, Yuling, Li, Tongtong, Huang, Tao, Liu, Hongbin. 2024. CWF19L2 is Essential for Male Fertility and Spermatogenesis by Regulating Alternative Splicing. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2403866. doi:10.1002/advs.202403866. https://pubmed.ncbi.nlm.nih.gov/38889293/
2. Chen, Lihong, Ye, Xiaoqi, Li, Yan, Ran, Xingwu. 2024. Systematic identification of therapeutic targets for coronary artery calcification: an integrated transcriptomic and proteomic Mendelian randomization. In Frontiers in cardiovascular medicine, 11, 1419440. doi:10.3389/fcvm.2024.1419440. https://pubmed.ncbi.nlm.nih.gov/39526184/
3. Nordgard, Silje H, Johansen, Fredrik E, Alnaes, Grethe I G, Børresen-Dale, Anne-Lise, Kristensen, Vessela N. . Genome-wide analysis identifies 16q deletion associated with survival, molecular subtypes, mRNA expression, and germline haplotypes in breast cancer patients. In Genes, chromosomes & cancer, 47, 680-96. doi:10.1002/gcc.20569. https://pubmed.ncbi.nlm.nih.gov/18398821/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen