C57BL/6NCya-Mtnr1bem1flox/Cya
Common Name:
Mtnr1b-flox
Product ID:
S-CKO-08625
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mtnr1b-flox
Strain ID
CKOCMP-244701-Mtnr1b-B6N-VA
Gene Name
Product ID
S-CKO-08625
Gene Alias
Mel-1B-R; Mel1b; Mt2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mtnr1bem1flox/Cya mice (Catalog S-CKO-08625) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000057920
NCBI RefSeq
NM_145712
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
MTNR1B, the gene encoding melatonin receptor 1B, is crucial in various biological processes. Melatonin, a neurohormone involved in circadian rhythms, binds to MTNR1B, and this interaction has implications in multiple pathways. Genetic variations in MTNR1B have been linked to glucose metabolism-related disorders, suggesting its importance in metabolic regulation [3,4].
In a Mtnr1b knockout mouse model, deletion of Mtnr1b disrupted placental angiogenesis via the VEGF signaling pathway, leading to fetal growth restriction. Specifically, Mtnr1b knockout increased placental oxidative stress and apoptosis, reduced angiogenesis and placental vascular density, and these changes were mediated by the disrupted STAT3/VEGFR2/PI3K/AKT pathway [1].
In another study, astrocyte-specific knockout of Mtnr1b in mice (Mtnr1b cKO𝐺𝑓𝑎𝑝 mice) resulted in anxiety-like behavior, caused by down-regulation of gamma-aminobutyric acid-ergic (GABAergic) synaptic function [2].
In conclusion, MTNR1B plays essential roles in placental function and neural behavior regulation. Mouse models with Mtnr1b knockout or conditional knockout have provided valuable insights into its functions in processes such as placental angiogenesis and neural synaptic function, which are relevant to diseases like fetal growth restriction and potentially neuropsychiatric disorders.
References：
1. Wang, Likai, Han, Qi, Yan, Laiqing, Zhang, Ran, Liu, Guoshi. 2024. Mtnr1b deletion disrupts placental angiogenesis through the VEGF signaling pathway leading to fetal growth restriction. In Pharmacological research, 206, 107290. doi:10.1016/j.phrs.2024.107290. https://pubmed.ncbi.nlm.nih.gov/38960012/
2. Meng, Zijun, Guo, Shipeng, Dong, Xiangjun, Zhou, Weihui, Song, Weihong. . Astrocyte-Ablation of Mtnr1b Increases Anxiety-Like Behavior in Adult Male Mice. In Journal of integrative neuroscience, 22, 154. doi:10.31083/j.jin2206154. https://pubmed.ncbi.nlm.nih.gov/38176947/
3. Garaulet, Marta, Qian, Jingyi, Florez, Jose C, Saxena, Richa, Scheer, Frank A J L. 2020. Melatonin Effects on Glucose Metabolism: Time To Unlock the Controversy. In Trends in endocrinology and metabolism: TEM, 31, 192-204. doi:10.1016/j.tem.2019.11.011. https://pubmed.ncbi.nlm.nih.gov/31901302/
4. Bonnefond, Amélie, Froguel, Philippe. 2017. Disentangling the Role of Melatonin and its Receptor MTNR1B in Type 2 Diabetes: Still a Long Way to Go? In Current diabetes reports, 17, 122. doi:10.1007/s11892-017-0957-1. https://pubmed.ncbi.nlm.nih.gov/29063374/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen