C57BL/6JCya-Laynem1flox/Cya
Common Name:
Layn-flox
Product ID:
S-CKO-08635
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Layn-flox
Strain ID
CKOCMP-244864-Layn-B6J-VA
Gene Name
Product ID
S-CKO-08635
Gene Alias
E030012M19Rik; Gm511
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Laynem1flox/Cya mice (Catalog S-CKO-08635) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098782
NCBI RefSeq
NM_001033534
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Layn, also known as Layilin, is an integral membrane hyaluronan receptor with a C-type lectin structural domain and is related to cell senescence [4]. It is involved in immune response pathways and has been associated with the regulation of T-cell function [3].
In hepatocellular carcinoma (HCC), Layn is highly expressed in tumors compared to peri-tumors, and high levels are associated with poor overall survival. It negatively regulates the immune function of CD8+ T cells, leading to their dysfunction [1].
In colorectal cancer, Layn is upregulated, and its abnormal expression is correlated with metastasis, poor prognosis, and TAM infiltration. oHA activates Layn, promoting metastasis and CCL20 secretion through the NF-kB pathway [2].
In gastric and colon cancers, high Layn expression is associated with poor survival, and it is positively correlated with infiltrating levels of various immune cells [3].
In conclusion, Layn plays a significant role in immune-related biological processes and is closely associated with the prognosis and immune infiltration in multiple cancers, especially hepatocellular, colorectal, gastric, and colon cancers. Studies on Layn enhance our understanding of tumor-immune interactions and may provide new targets for cancer immunotherapy [1,2,3].
References：
1. Xiao, Shuxiu, Lu, Lili, Lin, Zhiyuan, Wu, Weizhong, Zhou, Yuhong. 2024. LAYN Serves as a Prognostic Biomarker and Downregulates Tumor-Infiltrating CD8+ T Cell Function in Hepatocellular Carcinoma. In Journal of hepatocellular carcinoma, 11, 1031-1048. doi:10.2147/JHC.S464806. https://pubmed.ncbi.nlm.nih.gov/38859944/
2. Yang, Yabing, Chen, Zuyang, Chu, Xiaodong, Wang, Lu, Pan, Jinghua. 2023. Targeting LAYN inhibits colorectal cancer metastasis and tumor-associated macrophage infiltration induced by hyaluronan oligosaccharides. In Matrix biology : journal of the International Society for Matrix Biology, 117, 15-30. doi:10.1016/j.matbio.2023.02.005. https://pubmed.ncbi.nlm.nih.gov/36805871/
3. Pan, Jing-Hua, Zhou, Hong, Cooper, Laura, Pan, Yun-Long, Rong, Lijun. 2019. LAYN Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Gastric and Colon Cancers. In Frontiers in immunology, 10, 6. doi:10.3389/fimmu.2019.00006. https://pubmed.ncbi.nlm.nih.gov/30761122/
4. Jiawen, Wang, Jinfu, Wang, Jianyong, Liu, Yaoguang, Zhang, Jianye, Wang. 2023. Comprehensive landscape of the miRNA-regulated prognostic marker LAYN with immune infiltration and stemness in pan-cancer. In Journal of cancer research and clinical oncology, 149, 10989-11011. doi:10.1007/s00432-023-04986-7. https://pubmed.ncbi.nlm.nih.gov/37335337/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen