C57BL/6JCya-Gpr101em1flox/Cya
Common Name:
Gpr101-flox
Product ID:
S-CKO-08665
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpr101-flox
Strain ID
CKOCMP-245424-Gpr101-B6J-VA
Gene Name
Product ID
S-CKO-08665
Gene Alias
GPCR6; Gm365
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr101em1flox/Cya mice (Catalog S-CKO-08665) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000057645
NCBI RefSeq
NM_001033360
Target Region
Exon 2
Size of Effective Region
~5.4 kb
Detailed Document
Overview of Gene Research
Gpr101, an orphan G protein-coupled receptor belonging to Family A rhodopsin-like family, is predominantly expressed in the hypothalamus and pituitary. It plays a crucial role in regulating functions like energy balance and reproduction. Gpr101 couples to Gαs and Gαi proteins and is involved in the hypothalamus-pituitary-gonad axis. Its over-expression is linked to X-linked acrogigantism (XLAG) and acromegaly [1].
In transgenic mice with elevated somatotrope Gpr101 expression (GhrhrGpr101), Gpr101 causes elevated GH/prolactin secretion but without hyperplasia/tumorigenesis. It constitutively activates Gs, Gq/11, and G12/13, leading to GH secretion but not cell proliferation, similar to the signatures in human pituitary tumors with high Gpr101 expression [2].
In conclusion, Gpr101 is essential in regulating the somatotrope axis function. The study of Gpr101 transgenic mouse models has revealed its role in growth hormone hypersecretion and provided insights into the pathogenesis of XLAG and related pituitary diseases, contributing to a better understanding of these disease mechanisms.
References：
1. Hou, Zhi-Shuai, Tao, Ya-Xiong. 2018. Mutations in GPR101 as a potential cause of X-linked acrogigantism and acromegaly. In Progress in molecular biology and translational science, 161, 47-67. doi:10.1016/bs.pmbts.2018.10.003. https://pubmed.ncbi.nlm.nih.gov/30711029/
2. Abboud, Dayana, Daly, Adrian F, Dupuis, Nadine, Beckers, Albert, Hanson, Julien. 2020. GPR101 drives growth hormone hypersecretion and gigantism in mice via constitutive activation of Gs and Gq/11. In Nature communications, 11, 4752. doi:10.1038/s41467-020-18500-x. https://pubmed.ncbi.nlm.nih.gov/32958754/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen