C57BL/6JCya-Dkc1em1flox/Cya
Common Name:
Dkc1-flox
Product ID:
S-CKO-08670
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Dkc1-flox
Strain ID
CKOCMP-245474-Dkc1-B6J-VA
Gene Name
Product ID
S-CKO-08670
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dkc1em1flox/Cya mice (Catalog S-CKO-08670) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033776
NCBI RefSeq
NM_001030307
Target Region
Exon 4~5
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Dkc1, also known as dyskerin pseudouridine synthase 1, is associated with the formation of certain small RNAs and telomerase activity [5]. Mutations in DKC1 can lead to dyskeratosis congenita, a rare hereditary disease [3]. In normal biological processes, it may be involved in pathways related to cell proliferation, ribosome biogenesis, and telomere maintenance.
DKC1 has been found to play a significant role in multiple cancers. In colorectal cancer, DKC1 promotes cell proliferation by binding to and stabilizing the mRNA of several ribosomal proteins, and its knockdown attenuates cell growth. Dual inhibition of DKC1 and MEK1/2 synergistically restrains colorectal cancer cell growth [1].
In pancreatic ductal adenocarcinoma, SENP3 interacts with DKC1 and catalyzes its deSUMOylation, causing DKC1 instability and impairing cell migration. Overexpression of DKC1 abates the anti-metastasis effect of SENP3 [2].
In lung adenocarcinoma, DKC1 is overexpressed, and its knockdown induces G1 phase arrest, inhibits cell proliferation, and leads to telomere-related senescence and apoptosis [4].
In glioma, DKC1 expression is increased, and its knockdown inhibits cell growth, migration, and invasion [5].
In gastric cancer, DKC1 aggravates cell migration and invasion through up-regulating the expression of TNFAIP6 [6].
In neuroblastoma, DKC1 is highly expressed, and its knockout decreases cell activity, proliferation, invasion, and migration while increasing apoptosis [7]. A meta-analysis also shows that high DKC1 expression is predictive of worse prognosis and poorer clinicopathological parameters in malignancies [8].
In conclusion, Dkc1 is crucial for normal cellular functions, especially those related to ribosome production and telomerase complex function. Its dysregulation significantly impacts the development and progression of various cancers, such as colorectal, pancreatic, lung, glioma, gastric, and neuroblastoma cancers. The findings from loss-of-function experiments in these contexts have enhanced our understanding of Dkc1's role in disease, suggesting it could be a potential therapeutic target.
References：
1. Kan, Guangyan, Wang, Ziyang, Sheng, Chunjie, Mao, Yizhi, Chen, Shuai. 2021. Dual Inhibition of DKC1 and MEK1/2 Synergistically Restrains the Growth of Colorectal Cancer Cells. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8, 2004344. doi:10.1002/advs.202004344. https://pubmed.ncbi.nlm.nih.gov/34026451/
2. Wu, Xiao, Li, Jian-Hui, Xu, Long, Ni, Xuhao, Yin, Xiao-Yu. 2023. SUMO specific peptidase 3 halts pancreatic ductal adenocarcinoma metastasis via deSUMOylating DKC1. In Cell death and differentiation, 30, 1742-1756. doi:10.1038/s41418-023-01175-4. https://pubmed.ncbi.nlm.nih.gov/37188742/
3. AlSabbagh, Manahel Mahmood. 2020. Dyskeratosis congenita: a literature review. In Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG, 18, 943-967. doi:10.1111/ddg.14268. https://pubmed.ncbi.nlm.nih.gov/32930426/
4. Kan, Guangyan, Wang, Ziyang, Sheng, Chunjie, Mao, Yizhi, Chen, Shuai. 2021. Inhibition of DKC1 induces telomere-related senescence and apoptosis in lung adenocarcinoma. In Journal of translational medicine, 19, 161. doi:10.1186/s12967-021-02827-0. https://pubmed.ncbi.nlm.nih.gov/33879171/
5. Miao, Fa-An, Chu, Kun, Chen, Hai-Rong, Bai, Jin, You, Yong-Ping. 2019. Increased DKC1 expression in glioma and its significance in tumor cell proliferation, migration and invasion. In Investigational new drugs, 37, 1177-1186. doi:10.1007/s10637-019-00748-w. https://pubmed.ncbi.nlm.nih.gov/30847721/
6. Chen, Huihua, Wu, Yibo, Jiang, Yancheng, Chen, Zixuan, Zheng, Tingjin. 2024. DKC1 aggravates gastric cancer cell migration and invasion through up-regulating the expression of TNFAIP6. In Functional & integrative genomics, 24, 38. doi:10.1007/s10142-024-01313-2. https://pubmed.ncbi.nlm.nih.gov/38376551/
7. Wang, Xiao-Hui, Zhang, Shu-Feng, Wu, Hai-Ying, Wang, Xu-Hui, Gao, Tian-Hui. 2023. miRNA326-5p Targets DKC1 Gene to Regulate Apoptosis-Related Proteins and Intervene in the Development of Neuroblastoma. In Analytical cellular pathology (Amsterdam), 2023, 6761894. doi:10.1155/2023/6761894. https://pubmed.ncbi.nlm.nih.gov/37426487/
8. Zhang, Qin, Liu, Xiaohan, Zou, Zhenhong, Zhou, Bin. 2023. Evidence from a meta-analysis for the prognostic and clinicopathological importance of DKC1 in malignancies. In Future oncology (London, England), 19, 473-484. doi:10.2217/fon-2022-1125. https://pubmed.ncbi.nlm.nih.gov/36876511/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen