C57BL/6JCya-Vasnem1flox/Cya
Common Name:
Vasn-flox
Product ID:
S-CKO-08721
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Vasn-flox
Strain ID
CKOCMP-246154-Vasn-B6J-VA
Gene Name
Product ID
S-CKO-08721
Gene Alias
2610528G05Rik; Atia; Slitl2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vasnem1flox/Cya mice (Catalog S-CKO-08721) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038770
NCBI RefSeq
NM_139307
Target Region
Exon 2
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Vasn, which encodes vasorin, is a protein-coding gene involved in multiple biological processes. Vasorin is a transmembrane protein and is associated with several pathways, such as the YAP/TAZ, NOTCH, and MAPK pathways. It plays a crucial role in tumor development, angiogenesis, bone turnover, and vascular function [1-7, 9]. Genetic models, like knockout mice, are valuable for studying its functions.
In gene knockout studies, Vasn-deficient constitutive KO mice, but not osteoclast-targeted KO mice, showed an osteopenic bone phenotype due to enhanced osteoclast activity, indicating Vasn's role in bone turnover [7]. In myocardial fibrosis research, VASN-/-mice presented typical features of MF, with reduced extracellular matrix genes and up-regulated inflammatory factors, suggesting VASN knockout induces MF by regulating non-collagen fibers and inflammation [9]. In cancer research, knockdown of VASN in various cancer cell lines (prostate, colorectal, laryngeal, thyroid, and ARID1A-deficient lung adenocarcinoma) inhibited cell proliferation, migration, invasion, and in some cases, reversed epithelial-mesenchymal transition (EMT). This was often associated with the down-regulation of related signaling pathways, such as YAP/TAZ and NOTCH/MAPK [1,2,3,4,5,6]. In severe preeclampsia, decreased extracellular vesicle VASN was found to mediate endothelial dysfunction [8].
In conclusion, Vasn has diverse essential biological functions. Through gene knockout models, its roles in bone-related pathologies, myocardial fibrosis, cancer progression, and preeclampsia-related endothelial dysfunction have been revealed. These findings contribute to a better understanding of the underlying mechanisms of these diseases and may provide potential biomarker and therapeutic target candidates.
References:
1. Cui, F-L, Mahmud, A-N, Xu, Z-P, Wang, Z-Y, Hu, J-P. . VASN promotes proliferation of prostate cancer through the YAP/TAZ axis. In European review for medical and pharmacological sciences, 24, 6589-6596. doi:10.26355/eurrev_202006_21644. https://pubmed.ncbi.nlm.nih.gov/32633347/
2. Kang, Da, Huang, Shanshan, Liao, Yijun, Huang, Jingxiu, Zhang, R X. 2024. Vasorin (VASN) overexpression promotes pulmonary metastasis and resistance to adjuvant chemotherapy in patients with locally advanced rectal cancer. In Journal of translational medicine, 22, 742. doi:10.1186/s12967-024-05473-4. https://pubmed.ncbi.nlm.nih.gov/39107788/
3. Liang, Weiye, Zuo, Jia, Liu, Mingkai, Zhao, Jie, Wang, Jian. . VASN promotes colorectal cancer progression by activating the YAP/TAZ and AKT signaling pathways via YAP. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 37, e22688. doi:10.1096/fj.202201181R. https://pubmed.ncbi.nlm.nih.gov/36468780/
4. Liu, Haotian, Kong, Weili, Wen, Shuxin, Yin, Xiaoling, Liu, Yuhe. . VASN promotes proliferation of laryngeal cancer cells via YAP/TAZ. In Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 26, 1563-1570. doi:. https://pubmed.ncbi.nlm.nih.gov/34565020/
5. Bhandari, Adheesh, Guan, Yaoyao, Xia, Erjie, Huang, Qidi, Chen, Yizuo. 2019. VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro. In American journal of translational research, 11, 3589-3599. doi:. https://pubmed.ncbi.nlm.nih.gov/31312369/
6. Wu, Dan-Ni, Zhang, Kang-Liang, Chen, Rui-Heng, Zhao, Xiao-Dan, Huang, Ri-Sheng. 2024. VASN promotes the aggressive phenotype in ARID1A-deficient lung adenocarcinoma. In BMC cancer, 24, 1327. doi:10.1186/s12885-024-13083-y. https://pubmed.ncbi.nlm.nih.gov/39472811/
7. Andrique, Caroline, Bonnet, Anne Laure, Dang, Julien, Gaucher, Celine, Chaussain, Catherine. 2024. Vasorin as an actor of bone turnover? In Journal of cellular physiology, 239, e31257. doi:10.1002/jcp.31257. https://pubmed.ncbi.nlm.nih.gov/38504496/
8. Murugesan, Saravanakumar, Addis, Dylan R, Hussey, Hanna, Jilling, Tamas, Berkowitz, Dan E. 2024. Decreased Extracellular Vesicle Vasorin in Severe Preeclampsia Plasma Mediates Endothelial Dysfunction. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.06.24.600441. https://pubmed.ncbi.nlm.nih.gov/38979275/
9. Sun, Junming, Yin, Siwei, Li, Qiurui, Huang, Qiaojuan, He, Min. 2025. VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation. In Frontiers in pharmacology, 15, 1500617. doi:10.3389/fphar.2024.1500617. https://pubmed.ncbi.nlm.nih.gov/39898320/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen