C57BL/6JCya-Zfp277em1flox/Cya
Common Name:
Zfp277-flox
Product ID:
S-CKO-08725
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Zfp277-flox
Strain ID
CKOCMP-246196-Zfp277-B6J-VA
Gene Name
Product ID
S-CKO-08725
Gene Alias
2410017E24Rik; NIRF4; Znf277
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp277em1flox/Cya mice (Catalog S-CKO-08725) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069692
NCBI RefSeq
NM_172575
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Zfp277, also known as Zinc finger protein 277, is a transcription factor regulating cellular senescence. It is expressed in early stem cell progenitors and undifferentiated transit-amplifying cells (TACs) in the intestine and is involved in key cancer pathways such as β-catenin signaling [1]. It also interacts with polycomb group (PcG) proteins to regulate the Ink4a/Arf tumor suppressor gene locus [2].
In ApcMin/+ mice, Zfp277 deficiency attenuated intestinal epithelial cell proliferation and tumor formation, and prolonged survival. This indicates that Zfp277 acts as a colon cancer oncogene regulating cellular proliferation and senescence, in part by repressing p21WAF1 expression [1]. In mouse embryonic fibroblasts (MEFs), loss of Zfp277 caused dissociation of PcG proteins from the Ink4a/Arf locus, resulting in premature senescence [2].
In conclusion, Zfp277 is crucial in regulating cellular senescence and proliferation. Gene-knockout mouse models have revealed its role as an oncogene in colon cancer and its importance in the transcriptional regulation of the Ink4a/Arf locus. These findings contribute to understanding the mechanisms of cancer development and cellular senescence.
References：
1. Xie, Guofeng, Peng, Zhongsheng, Liang, Jinqing, Yfantis, Harris, Raufman, Jean-Pierre. 2022. Zinc finger protein 277 is an intestinal transit-amplifying cell marker and colon cancer oncogene. In JCI insight, 7, . doi:10.1172/jci.insight.150894. https://pubmed.ncbi.nlm.nih.gov/35015732/
2. Negishi, Masamitsu, Saraya, Atsunori, Mochizuki, Shinobu, Koseki, Haruhiko, Iwama, Atsushi. 2010. A novel zinc finger protein Zfp277 mediates transcriptional repression of the Ink4a/arf locus through polycomb repressive complex 1. In PloS one, 5, e12373. doi:10.1371/journal.pone.0012373. https://pubmed.ncbi.nlm.nih.gov/20808772/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen