C57BL/6JCya-Mpstem1flox/Cya
Common Name:
Mpst-flox
Product ID:
S-CKO-08727
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mpst-flox
Strain ID
CKOCMP-246221-Mpst-B6J-VA
Gene Name
Product ID
S-CKO-08727
Gene Alias
Mst
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mpstem1flox/Cya mice (Catalog S-CKO-08727) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043865
NCBI RefSeq
NM_138670
Target Region
Exon 2
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Mpst, or 3-mercaptopyruvate sulfurtransferase, is a mitochondrial cysteine-catabolizing enzyme. It is involved in multiple biological functions such as hydrogen sulfide (H₂S) generation, protein S-persulfidation, mitochondrial protein import, and bioenergetics regulation [2,4,5]. It participates in pathways related to inflammation, metabolism, and cell apoptosis, playing a significant role in maintaining physiological homeostasis. Genetic models like gene knockout (KO) mice are valuable for studying its functions.
In Mpst-deficient mice, fat accumulation occurs when fed a high-fat diet, along with increased body weight, reduced metabolic rate, and impaired glucose/insulin tolerance, suggesting its role in regulating adipose tissue biology and metabolic health [2]. In colitis models, Mpst deficiency promotes intestinal epithelial cell apoptosis, aggravates inflammatory responses, and worsens the symptoms of murine colitis, likely through regulating the AKT/apoptosis axis [1]. Double ablation of Cth/Mpst in mice leads to enhanced vasorelaxation and reduced blood pressure via upregulation of the eNOS/sGC pathway [3].
In conclusion, Mpst is essential for maintaining mitochondrial function, regulating metabolism, and protecting against inflammation and apoptosis. Studies using Mpst KO mouse models have revealed its significant roles in obesity, inflammatory bowel disease, and cardiovascular function, providing potential therapeutic targets for these diseases.
References:
1. Zhang, Jie, Cen, Li, Zhang, Xiaofen, Wu, Hao, Shen, Zhe. 2022. MPST deficiency promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via AKT. In Redox biology, 56, 102469. doi:10.1016/j.redox.2022.102469. https://pubmed.ncbi.nlm.nih.gov/36126419/
2. Katsouda, Antonia, Valakos, Dimitrios, Dionellis, Vasilios S, Szabo, Csaba, Papapetropoulos, Andreas. 2022. MPST sulfurtransferase maintains mitochondrial protein import and cellular bioenergetics to attenuate obesity. In The Journal of experimental medicine, 219, . doi:10.1084/jem.20211894. https://pubmed.ncbi.nlm.nih.gov/35616614/
3. Katsouda, Antonia, Markou, Maria, Zampas, Paraskevas, Bucci, Mariarosaria, Papapetropoulos, Andreas. 2023. CTH/MPST double ablation results in enhanced vasorelaxation and reduced blood pressure via upregulation of the eNOS/sGC pathway. In Frontiers in pharmacology, 14, 1090654. doi:10.3389/fphar.2023.1090654. https://pubmed.ncbi.nlm.nih.gov/36860295/
4. Pedre, Brandán, Talwar, Deepti, Barayeu, Uladzimir, Glatt, Sebastian, Dick, Tobias P. 2023. 3-Mercaptopyruvate sulfur transferase is a protein persulfidase. In Nature chemical biology, 19, 507-517. doi:10.1038/s41589-022-01244-8. https://pubmed.ncbi.nlm.nih.gov/36732619/
5. Pedre, Brandán, Dick, Tobias P. 2020. 3-Mercaptopyruvate sulfurtransferase: an enzyme at the crossroads of sulfane sulfur trafficking. In Biological chemistry, 402, 223-237. doi:10.1515/hsz-2020-0249. https://pubmed.ncbi.nlm.nih.gov/33055309/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen