C57BL/6JCya-Oas2em1flox/Cya
Common Name:
Oas2-flox
Product ID:
S-CKO-08745
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Oas2-flox
Strain ID
CKOCMP-246728-Oas2-B6J-VA
Gene Name
Product ID
S-CKO-08745
Gene Alias
Oasl11
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Oas2em1flox/Cya mice (Catalog S-CKO-08745) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081491
NCBI RefSeq
NM_001347448.1
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Oas2, a member of the 2'-5'-oligoadenylate synthetases (OAS) family, is an interferon (IFN)-induced antiviral enzyme [1,2,3,4,6,7,8,9]. It generates 2'-5'-linked oligoadenylates upon activation by double-stranded RNA, which can activate ribonuclease L to degrade single-stranded RNA. This process is involved in the antiviral response system and can also influence the IFN-activated Jak/STAT signaling pathway [3,7].
In breast cancer, high mRNA expression of Oas2 is associated with favorable prognosis, while in psoriasis, its overexpression is found in lesional skin and serum, and it contributes to epidermal keratinocyte proliferation by regulating the cell cycle and augmenting IFN-1-induced phosphorylation of Jak1 and signal transducer and activator of transcription 1 [1,2]. Inborn errors of Oas2 in children are related to SARS-CoV-2-related multisystem inflammatory syndrome (MIS-C), where Oas2-deficient monocytic cell lines produce excessive inflammatory cytokines upon dsRNA or SARS-CoV-2 stimulation [3]. Activation of Oas2 in a mouse model with an activating mutation prevented pregnancy-driven mammary cancer metastases and enhanced the effectiveness of checkpoint immunotherapy [4]. In testicular cancer, upregulating Oas2 by piR-36249 and DHX36 inhibits cancer cell progression [5]. Oas2 also restricts intracellular Mycobacterium tuberculosis replication, enhances pro-inflammatory cytokine secretion, and inhibits Zika virus replication through activation of the type Ι IFN signaling pathway [6,7]. In COVID-19, genetic variants in the gene cluster encoding Oas2 are associated with critical illness [8]. The activity of Oas2 is affected by the characteristics of double-stranded RNA, with a minimum dsRNA length requirement of 35 bp for activation [9]. In psoriasis, Oas2 is a potential biomarker for disease activity [10].
In conclusion, Oas2 plays crucial roles in multiple biological processes and disease conditions. Its functions range from antiviral responses to influencing cell proliferation, metastasis, and inflammation in various diseases such as cancer, psoriasis, and COVID-19. Studies using genetic models, especially in mice, have provided valuable insights into these functions, helping to understand the underlying mechanisms and potentially guiding the development of therapeutic strategies.
References:
1. Zhang, Yujie, Yu, Chaoran. 2020. Prognostic characterization of OAS1/OAS2/OAS3/OASL in breast cancer. In BMC cancer, 20, 575. doi:10.1186/s12885-020-07034-6. https://pubmed.ncbi.nlm.nih.gov/32560641/
2. Huang, Yan-Zhou, Zheng, Yu-Xin, Zhou, Yuan, Zheng, Min, Man, Xiao-Yong. 2022. OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augmenting IFN-1‒Induced Jak1‒Signal Transducer and Activator of Transcription 1 Phosphorylation in Psoriasis. In The Journal of investigative dermatology, 142, 2635-2645.e9. doi:10.1016/j.jid.2022.02.018. https://pubmed.ncbi.nlm.nih.gov/35305973/
3. Lee, Danyel, Le Pen, Jérémie, Yatim, Ahmad, Zhang, Shen-Ying, Casanova, Jean-Laurent. 2023. Inborn errors of OAS-RNase L in SARS-CoV-2-related multisystem inflammatory syndrome in children. In Science (New York, N.Y.), 379, eabo3627. doi:10.1126/science.abo3627. https://pubmed.ncbi.nlm.nih.gov/36538032/
4. Ho, Wing-Hong Jonathan, Law, Andrew M K, Masle-Farquhar, Etienne, Oakes, Samantha R, Ormandy, Christopher J. 2022. Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases. In Breast cancer research : BCR, 24, 31. doi:10.1186/s13058-022-01525-z. https://pubmed.ncbi.nlm.nih.gov/35505346/
5. Wang, Qianqian, Chen, Peize, Wang, Xiaorong, Song, Xiaoyuan, Sun, Fei. 2023. piR-36249 and DHX36 together inhibit testicular cancer cells progression by upregulating OAS2. In Non-coding RNA research, 8, 174-186. doi:10.1016/j.ncrna.2022.12.004. https://pubmed.ncbi.nlm.nih.gov/36710986/
6. Leisching, Gina, Cole, Victoria, Ali, Aus T, Baker, Bienyameen. 2019. OAS1, OAS2 and OAS3 restrict intracellular M. tb replication and enhance cytokine secretion. In International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 80S, S77-S84. doi:10.1016/j.ijid.2019.02.029. https://pubmed.ncbi.nlm.nih.gov/30822544/
7. Liao, Xinzhong, Xie, He, Li, Shilin, Yang, Chunhui, Chen, Limin. 2020. 2', 5'-Oligoadenylate Synthetase 2 (OAS2) Inhibits Zika Virus Replication through Activation of Type Ι IFN Signaling Pathway. In Viruses, 12, . doi:10.3390/v12040418. https://pubmed.ncbi.nlm.nih.gov/32276512/
8. Pairo-Castineira, Erola, Clohisey, Sara, Klaric, Lucija, Wilson, James F, Baillie, J Kenneth. 2020. Genetic mechanisms of critical illness in COVID-19. In Nature, 591, 92-98. doi:10.1038/s41586-020-03065-y. https://pubmed.ncbi.nlm.nih.gov/33307546/
9. Koul, Amit, Deo, Soumya, Booy, Evan P, Genung, Matthew, McKenna, Sean A. 2019. Impact of double-stranded RNA characteristics on the activation of human 2'-5'-oligoadenylate synthetase 2 (OAS2). In Biochemistry and cell biology = Biochimie et biologie cellulaire, 98, 70-82. doi:10.1139/bcb-2019-0060. https://pubmed.ncbi.nlm.nih.gov/30965010/
10. Zhou, Yuan, Wang, Ping, Yan, Bing-Xi, Zheng, Min, Man, Xiao-Yong. 2020. Quantitative Proteomic Profile of Psoriatic Epidermis Identifies OAS2 as a Novel Biomarker for Disease Activity. In Frontiers in immunology, 11, 1432. doi:10.3389/fimmu.2020.01432. https://pubmed.ncbi.nlm.nih.gov/32849499/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen