C57BL/6JCya-Klk8em1flox/Cya
Common Name:
Klk8-flox
Product ID:
S-CKO-09510
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Klk8-flox
Strain ID
CKOCMP-259277-Klk8-B6J-VA
Gene Name
Product ID
S-CKO-09510
Gene Alias
BSP1; NP; Nrpn; Prss19
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Klk8em1flox/Cya mice (Catalog S-CKO-09510) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000085461
NCBI RefSeq
NM_008940
Target Region
Exon 2~3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Klk8, also known as tissue kallikrein-related peptidase 8, is a tryptic serine protease with limited substrate specificity. It is involved in various biological processes. In the brain, it participates in activity-dependent synaptic changes like long-term potentiation. In the skin, it might be involved in desquamation through degrading epidermal adhesive molecules. It is also associated with multiple signaling pathways such as PI3K-Akt-mTOR, Notch, and EGF-dependent PI3K/Akt/mTOR in cancer-related contexts [2,3].
Genetic models, especially Klk8 knockout (KO) mouse models, have provided insights into its functions. In a CUMS-induced depression rodent model, Klk8 deficiency attenuated depression-like behaviors and hippocampal neuronal apoptosis, suggesting Klk8 upregulation promotes apoptosis via cleaving NCAM1 [1]. In TgCRND8 mice, genetic Klk8-knockdown led to a significant decline of amyloid beta (Aβ) and tau pathology in a sex-specific manner, improving structural neuroplasticity and memory performance, reinforcing its potential as a therapeutic target in Alzheimer's disease [4]. In diabetic mice, Klk8 deficiency alleviated diabetic cardiac fibrosis and rescued cardiac function, uncovering a pro-EndMT mechanism in diabetic cardiac fibrosis via Klk8 upregulation [5]. In hypoxia-induced H9c2 cardiomyocytes, Klk8 silencing attenuated cardiomyocyte hypertrophy, indicating its role in regulating right ventricular hypertrophy in hypoxic pulmonary hypertension [6].
In conclusion, Klk8 is a multifunctional protease involved in tissue development, synaptic changes, and disease-related processes. The study of Klk8 KO mouse models has significantly contributed to understanding its role in diseases such as depression, Alzheimer's disease, diabetic cardiomyopathy, and hypoxic pulmonary hypertension-related right ventricular hypertrophy, providing potential therapeutic targets for these conditions.
References:
1. Xu, Dan-Hong, Du, Jian-Kui, Liu, Shi-Yu, Zhu, Xiao-Yan, Liu, Yu-Jian. 2023. Upregulation of KLK8 contributes to CUMS-induced hippocampal neuronal apoptosis by cleaving NCAM1. In Cell death & disease, 14, 278. doi:10.1038/s41419-023-05800-5. https://pubmed.ncbi.nlm.nih.gov/37076499/
2. Yoshida, Shigetaka. . Klk8, a multifunctional protease in the brain and skin: analysis of knockout mice. In Biological chemistry, 391, 375-80. doi:10.1515/BC.2010.034. https://pubmed.ncbi.nlm.nih.gov/20180635/
3. Hua, Qing, Li, Tianjiao, Liu, Yixuan, Zhu, Xiaoyan, Xu, Pingbo. 2021. Upregulation of KLK8 Predicts Poor Prognosis in Pancreatic Cancer. In Frontiers in oncology, 11, 624837. doi:10.3389/fonc.2021.624837. https://pubmed.ncbi.nlm.nih.gov/34395235/
4. Herring, Arne, Kurapati, Nirup K, Krebs, Sofia, Münster, Yvonne, Keyvani, Kathy. 2021. Genetic knockdown of Klk8 has sex-specific multi-targeted therapeutic effects on Alzheimer's pathology in mice. In Neuropathology and applied neurobiology, 47, 611-624. doi:10.1111/nan.12687. https://pubmed.ncbi.nlm.nih.gov/33341972/
5. Du, Jian-Kui, Yu, Qing, Liu, Yu-Jian, Ni, Xin, Zhu, Xiao-Yan. 2021. A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis. In Theranostics, 11, 4207-4231. doi:10.7150/thno.48530. https://pubmed.ncbi.nlm.nih.gov/33754057/
6. Sun, Haixia, Li, Jia, Wang, Qinggong, Song, Meilin, Feng, Jinhua. 2022. Kallikrein-related peptidase-8 (KLK8) aggravated hypoxia-induced right ventricular hypertrophy by targeting P38 MAPK/P53 signaling pathway. In Tissue & cell, 78, 101874. doi:10.1016/j.tice.2022.101874. https://pubmed.ncbi.nlm.nih.gov/35994918/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen