C57BL/6JCya-Cdk8em1flox/Cya
Common Name:
Cdk8-flox
Product ID:
S-CKO-09567
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdk8-flox
Strain ID
CKOCMP-264064-Cdk8-B6J-VA
Gene Name
Product ID
S-CKO-09567
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdk8em1flox/Cya mice (Catalog S-CKO-09567) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031640
NCBI RefSeq
NM_153599
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Cdk8, or cyclin-dependent kinase 8, is a key member of the cyclin-dependent kinase family. It is associated with transcription regulation, acting as a "molecular switch" in the Mediator complex. Cdk8 phosphorylates transcription factors to regulate gene expression and can control the transcription process via the Mediator complex. It has been implicated in multiple biological processes and is considered a potential drug target for diseases like breast and prostate cancer [1,3,5,7].
In Drosophila, neuronal loss of Cdk8 severely reduces fly lifespan and causes bang sensitivity. Cdk8 promotes mitochondrial fission through Drp1 phosphorylation at S616. Intriguingly, overexpression of Cdk8 can suppress phenotypes in flies with low levels of Pink1, a mitochondrial kinase involved in Parkinson's disease [2]. In T cells, inhibition of Cdk8 and Cdk19 can convert antigen-specific effector/memory T cells into Foxp3-expressing Treg cells, suggesting its role in immunological diseases [4]. Cdk8 inhibitors can also antagonize HIV-1 reactivation and promote provirus latency in T cells [6].
In summary, Cdk8 is crucial for transcription-related processes, mitochondrial fission, and immune-related functions. Its study in models like Drosophila has provided insights into its role in lifespan, neurodegenerative-like phenotypes, and immunological and viral-related diseases. These findings suggest Cdk8 as a potential target for treating cancer, immunological disorders, and HIV-1-related conditions [1-4,9,10].
References:
1. Menzl, Ingeborg, Witalisz-Siepracka, Agnieszka, Sexl, Veronika. 2019. CDK8-Novel Therapeutic Opportunities. In Pharmaceuticals (Basel, Switzerland), 12, . doi:10.3390/ph12020092. https://pubmed.ncbi.nlm.nih.gov/31248103/
2. Liao, Jenny Zhe, Chung, Hyung-Lok, Shih, Claire, Bellen, Hugo J, Verheyen, Esther M. 2024. Cdk8/CDK19 promotes mitochondrial fission through Drp1 phosphorylation and can phenotypically suppress pink1 deficiency in Drosophila. In Nature communications, 15, 3326. doi:10.1038/s41467-024-47623-8. https://pubmed.ncbi.nlm.nih.gov/38637532/
3. Wu, Dan, Zhang, Zhaoyan, Chen, Xing, Yan, Yaoyao, Liu, Xinhua. 2020. Angel or Devil ? - CDK8 as the new drug target. In European journal of medicinal chemistry, 213, 113043. doi:10.1016/j.ejmech.2020.113043. https://pubmed.ncbi.nlm.nih.gov/33257171/
4. Akamatsu, Masahiko, Mikami, Norihisa, Ohkura, Naganari, Narumiya, Shuh, Sakaguchi, Shimon. . Conversion of antigen-specific effector/memory T cells into Foxp3-expressing Treg cells by inhibition of CDK8/19. In Science immunology, 4, . doi:10.1126/sciimmunol.aaw2707. https://pubmed.ncbi.nlm.nih.gov/31653719/
5. Rzymski, Tomasz, Mikula, Michał, Wiklik, Katarzyna, Brzózka, Krzysztof. 2015. CDK8 kinase--An emerging target in targeted cancer therapy. In Biochimica et biophysica acta, 1854, 1617-29. doi:10.1016/j.bbapap.2015.05.011. https://pubmed.ncbi.nlm.nih.gov/26006748/
6. Horvath, Riley M, Brumme, Zabrina L, Sadowski, Ivan. 2023. CDK8 inhibitors antagonize HIV-1 reactivation and promote provirus latency in T cells. In Journal of virology, 97, e0092323. doi:10.1128/jvi.00923-23. https://pubmed.ncbi.nlm.nih.gov/37671866/
7. Yin, Xiaomin, He, Zhilong, Chen, Kun, Tang, Hailin, Cai, Manbo. 2024. Unveiling the impact of CDK8 on tumor progression: mechanisms and therapeutic strategies. In Frontiers in pharmacology, 15, 1386929. doi:10.3389/fphar.2024.1386929. https://pubmed.ncbi.nlm.nih.gov/38606172/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen