C57BL/6JCya-Map3k8em1flox/Cya
Common Name:
Map3k8-flox
Product ID:
S-CKO-09571
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Map3k8-flox
Strain ID
CKOCMP-26410-Map3k8-B6J-VA
Gene Name
Product ID
S-CKO-09571
Gene Alias
Cot; Cot/Tpl2; Est; Estf; Tpl-2; Tpl2; c-COT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Map3k8em1flox/Cya mice (Catalog S-CKO-09571) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025078
NCBI RefSeq
NM_007746
Target Region
Exon 3~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Map3k8, also known as Cot/Tpl2, is a serine/threonine kinase. It activates the MKK1/2-ERK1/2 MAPK pathway downstream from multiple receptors such as interleukin-1R, tumor necrosis factor-αR, etc. It plays a key role in innate and adaptive immunity, and influences various inflammatory processes and is involved in regulating cell functions [2].
In gene-knockout (KO) mouse models, Map3k8 deficiency led to smaller numbers of certain monocyte subtypes in ApoE-/-mice fed a high-fat diet, with monocytes showing high apoptosis rates and irregular cytokine expression, suggesting its role in atherogenesis [2]. In lipopolysaccharide-induced emergency granulopoiesis, Map3k8-/-mice had decreased production of granulocyte colony-stimulating factor (G-CSF) and impaired neutrophil precursor proliferation, indicating its requirement for this process [3]. In the context of pulmonary fibrosis, Map3k8 mRNA levels were decreased in IPF patients and in a mouse model of pulmonary fibrosis. Ubiquitous or macrophage-specific deletion of Map3k8 exacerbated the disease, showing its role in regulating inflammatory responses and Cox-2-mediated PGE2 production in the lung [1].
In conclusion, Map3k8 is crucial for regulating immune and inflammatory responses. Studies using KO mouse models have revealed its significant contributions in diseases such as atherogenesis, pulmonary fibrosis, and emergency granulopoiesis, providing insights into potential therapeutic strategies targeting Map3k8 in these disease areas.
References:
1. Zannikou, Markella, Barbayianni, Ilianna, Fanidis, Dionysios, Kaffe, Eleanna, Aidinis, Vassilis. 2020. MAP3K8 Regulates Cox-2-Mediated Prostaglandin E2 Production in the Lung and Suppresses Pulmonary Inflammation and Fibrosis. In Journal of immunology (Baltimore, Md. : 1950), 206, 607-620. doi:10.4049/jimmunol.2000862. https://pubmed.ncbi.nlm.nih.gov/33443087/
2. Sanz-Garcia, Carlos, Sánchez, Ángela, Contreras-Jurado, Constanza, Aranda, Ana, Alemany, Susana. 2016. Map3k8 Modulates Monocyte State and Atherogenesis in ApoE-/- Mice. In Arteriosclerosis, thrombosis, and vascular biology, 37, 237-246. doi:10.1161/ATVBAHA.116.308528. https://pubmed.ncbi.nlm.nih.gov/27856455/
3. Sánchez, Ángela, Relaño, Carlos, Carrasco, Araceli, Aranda, Ana, Alemany, Susana. 2017. Map3k8 controls granulocyte colony-stimulating factor production and neutrophil precursor proliferation in lipopolysaccharide-induced emergency granulopoiesis. In Scientific reports, 7, 5010. doi:10.1038/s41598-017-04538-3. https://pubmed.ncbi.nlm.nih.gov/28694430/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen