C57BL/6JCya-Slc38a9em1flox/Cya
Common Name:
Slc38a9-flox
Product ID:
S-CKO-09683
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc38a9-flox
Strain ID
CKOCMP-268706-Slc38a9-B6J-VA
Gene Name
Product ID
S-CKO-09683
Gene Alias
4833412L08Rik; 6720411P22Rik; 9130023D20Rik; 9430067K09Rik; A730092C09
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc38a9em1flox/Cya mice (Catalog S-CKO-09683) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052514
NCBI RefSeq
NM_178746
Target Region
Exon 3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Slc38a9, a lysosomal membrane protein homologous to amino acid transporters, is also known as a transceptor [4]. It plays a crucial role in amino acid homeostasis and is an essential component in the mTORC1 signaling pathway. mTORC1, a master growth regulator, senses various environmental cues including amino acids, and SLC38A9 is required for arginine-mediated activation of mTORC1 [1]. It also mediates the transport of many essential amino acids out of lysosomes in an arginine-regulated manner, which is vital for cell growth [1].
In zebrafish, slc38a9 -/- mutants showed increased apoptosis, dysregulated amino acid metabolism, and glycolysis/gluconeogenesis disorders [3]. A quarter of the mutant embryos developed pericardial edema and died prematurely. These findings suggest that Slc38a9 is indispensable for embryonic development, metabolic regulation, and stress adaption [3]. In pancreatic cancer cells, which rely on macropinocytosed protein as a nutrient source, SLC38A9 is required to form tumors, indicating its role in cancer-related metabolic processes [1]. Also, SLC38A9 knockdown prevents S1-induced endolysosome de-acidification and blocks the S protein-mediated entry of pseudo-SARS-CoV-2 in multiple cell lines, revealing its function in regulating viral entry [2].
In conclusion, Slc38a9 is essential for maintaining amino acid homeostasis and regulating the mTORC1 signaling pathway. Model-based research, such as the zebrafish slc38a9 knockout model, has revealed its crucial roles in embryonic development, metabolism, and disease-related processes like cancer growth and viral entry. Understanding the function of Slc38a9 provides insights into the mechanisms of these biological processes and potential therapeutic targets for related diseases.
References:
1. Wyant, Gregory A, Abu-Remaileh, Monther, Wolfson, Rachel L, Vander Heiden, Matthew G, Sabatini, David M. . mTORC1 Activator SLC38A9 Is Required to Efflux Essential Amino Acids from Lysosomes and Use Protein as a Nutrient. In Cell, 171, 642-654.e12. doi:10.1016/j.cell.2017.09.046. https://pubmed.ncbi.nlm.nih.gov/29053970/
2. Datta, Gaurav, Rezagholizadeh, Neda, Hasler, Wendie A, Khan, Nabab, Chen, Xuesong. 2024. SLC38A9 regulates SARS-CoV-2 viral entry. In iScience, 27, 110387. doi:10.1016/j.isci.2024.110387. https://pubmed.ncbi.nlm.nih.gov/39071889/
3. Wu, Xiya, Chen, Jianyang, Liu, Chengdong, Mai, Kangsen, He, Gen. 2022. Slc38a9 Deficiency Induces Apoptosis and Metabolic Dysregulation and Leads to Premature Death in Zebrafish. In International journal of molecular sciences, 23, . doi:10.3390/ijms23084200. https://pubmed.ncbi.nlm.nih.gov/35457018/
4. Scalise, Mariafrancesca, Galluccio, Michele, Pochini, Lorena, Superti-Furga, Giulio, Indiveri, Cesare. 2019. Insights into the transport side of the human SLC38A9 transceptor. In Biochimica et biophysica acta. Biomembranes, 1861, 1558-1567. doi:10.1016/j.bbamem.2019.07.006. https://pubmed.ncbi.nlm.nih.gov/31295473/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen