C57BL/6JCya-Nlrc3em1flox/Cya
Common Name:
Nlrc3-flox
Product ID:
S-CKO-09706
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nlrc3-flox
Strain ID
CKOCMP-268857-Nlrc3-B6J-VA
Gene Name
Product ID
S-CKO-09706
Gene Alias
CLR16.2; D230007K08Rik; mFLJ00348
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nlrc3em1flox/Cya mice (Catalog S-CKO-09706) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000177551
NCBI RefSeq
NM_001081280
Target Region
Exon 2~3
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
NLRC3, a member of the pattern recognition receptors nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) family, is an important regulator of innate immune system homeostasis [3,4]. It can inhibit excessive immune response in the body by influencing multiple signaling pathways, such as NF-κB, STING/TBK1, and inflammasome-related pathways. It also plays roles in cell fate regulation, like preventing proliferation, promoting apoptosis, and inhibiting pyroptosis [3].
NLRC3 deficiency promotes hypoxia-induced pulmonary hypertension development via the IKK/NF-κB p65/HIF-1α pathway. In hypoxia-induced mice models, NLRC3 knockout led to increased right ventricular systolic pressure, right ventricular hypertrophy and fibrosis. In vitro and in vivo, it promoted PASMCs proliferation, HUVECs apoptosis, migration and inflammation [1].
Myeloid-specific NLRC3 deletion in mice improved macrophage glycolysis and sepsis-induced immunosuppression, as NLRC3 was found to inhibit the binding of NF-κB p65 to NFAT5, controlling the expression of glycolytic genes and pro-inflammatory cytokines in immunosuppressive macrophages [2].
Nlrc3 knockout mice showed accelerated cutaneous wound healing, mainly due to its regulatory effects on p53 signaling. Nlrc3 was found to mediate the ubiquitination and degradation of p53 in an Hsp90-dependent manner [5].
After HTNV infection, Nlrc3-/-mice developed weight loss, renal hemorrhage, and tubule dilation, resembling human HFRS symptoms, with higher viral loads in serum, spleen, and kidney compared to wild-type mice [6].
In conclusion, NLRC3 plays a crucial role in regulating immune responses, cell proliferation, and apoptosis, and its dysregulation is associated with various diseases including pulmonary hypertension, sepsis, wound healing, and HTNV-induced renal diseases. The use of NLRC3 knockout mouse models has provided valuable insights into its functions in these disease-related biological processes, facilitating a better understanding of disease mechanisms and potential therapeutic targets.
References：
1. Maimaitiaili, Nuerbiyemu, Zeng, Yanxi, Ju, Peinan, Zhuoga, Deji, Yu, Qing. 2023. NLRC3 deficiency promotes hypoxia-induced pulmonary hypertension development via IKK/NF-κB p65/HIF-1α pathway. In Experimental cell research, 431, 113755. doi:10.1016/j.yexcr.2023.113755. https://pubmed.ncbi.nlm.nih.gov/37586455/
2. Xu, Jiqian, Gao, Chenggang, He, Yajun, Yao, Shanglong, Shang, You. 2022. NLRC3 expression in macrophage impairs glycolysis and host immune defense by modulating the NF-κB-NFAT5 complex during septic immunosuppression. In Molecular therapy : the journal of the American Society of Gene Therapy, 31, 154-173. doi:10.1016/j.ymthe.2022.08.023. https://pubmed.ncbi.nlm.nih.gov/36068919/
3. Sun, Deyi, Xu, Jiqian, Zhang, Wanying, He, Yajun, Shang, You. 2022. Negative regulator NLRC3: Its potential role and regulatory mechanism in immune response and immune-related diseases. In Frontiers in immunology, 13, 1012459. doi:10.3389/fimmu.2022.1012459. https://pubmed.ncbi.nlm.nih.gov/36341336/
4. Zhao, Yue, Li, Ruiting. 2022. Overview of the anti-inflammatory function of the innate immune sensor NLRC3. In Molecular immunology, 153, 36-41. doi:10.1016/j.molimm.2022.11.014. https://pubmed.ncbi.nlm.nih.gov/36403432/
5. Qin, Yuan, Wu, Kai, Zhang, Zheng, Lu, Liting, Lu, Xincheng. 2022. NLRC3 deficiency promotes cutaneous wound healing due to the inhibition of p53 signaling. In Biochimica et biophysica acta. Molecular basis of disease, 1868, 166518. doi:10.1016/j.bbadis.2022.166518. https://pubmed.ncbi.nlm.nih.gov/35963285/
6. Ma, Ruixue, Zhang, Xiaoxiao, Shu, Jiayi, Liu, Rongrong, Wu, Xingan. 2021. Nlrc3 Knockout Mice Showed Renal Pathological Changes After HTNV Infection. In Frontiers in immunology, 12, 692509. doi:10.3389/fimmu.2021.692509. https://pubmed.ncbi.nlm.nih.gov/34335602/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen