C57BL/6JCya-Abatem1flox/Cya
Common Name:
Abat-flox
Product ID:
S-CKO-09709
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Abat-flox
Strain ID
CKOCMP-268860-Abat-B6J-VA
Gene Name
Product ID
S-CKO-09709
Gene Alias
9630038C02Rik; Gabaat; Gabat; Gm9851; I54; Laibat
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Abatem1flox/Cya mice (Catalog S-CKO-09709) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000065987
NCBI RefSeq
NM_172961
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
ABAT, also known as GABA-transaminase, is a key enzyme in the metabolism of gamma-aminobutyric acid (GABA), the most important inhibitory neurotransmitter in the brain. GABA metabolism is crucial for maintaining the balance between neuronal excitation and inhibition, and thus plays a significant role in normal brain function [5]. Mutations in ABAT can lead to GABA-transaminase deficiency, resulting in encephalopathy with symptoms like hypersomnolence, hypotonia, hypomyelination, and seizures [3].
In liver cancer, inactivating ABAT was associated with poor prognosis, and overexpressing ABAT could inhibit cancer cell behaviors and suppress tumorigenesis in nude mice. It was also found that miR-183-5p could target and downregulate ABAT expression in liver cancer [1].
In myelodysplastic syndrome, ABAT gene interference increased the sensitivity of cells to hypomethylating agents treatment, while ABAT overexpression decreased its sensitivity. The downregulation of ABAT gene expression could activate the CXCR4/mTOR signaling pathway, which was related to the sensitivity of hypomethylating agents [2].
In clear cell renal cell carcinoma, downregulated ABAT expression was observed, and overexpression of ABAT significantly attenuated cell proliferation and migration [4].
In conclusion, ABAT is essential for GABA metabolism and normal brain function. Its dysregulation is implicated in multiple diseases, including liver cancer, myelodysplastic syndrome, and clear cell renal cell carcinoma. Studies using in vivo models like nude mice in liver cancer research, and cell-based functional experiments in myelodysplastic syndrome and clear cell renal cell carcinoma, have provided insights into the role of ABAT in disease processes, highlighting its potential as a therapeutic target.
References:
1. Han, Hui, Zhou, Shenkang, Chen, Gengzhen, Lu, Yandi, Lin, Hui. 2021. ABAT targeted by miR-183-5p regulates cell functions in liver cancer. In The international journal of biochemistry & cell biology, 141, 106116. doi:10.1016/j.biocel.2021.106116. https://pubmed.ncbi.nlm.nih.gov/34742920/
2. Zhao, Guangjie, Li, Shuang, Wang, Qian, Wang, Wei, Wang, Xiaoqin. 2022. ABAT gene expression associated with the sensitivity of hypomethylating agents in myelodysplastic syndrome through CXCR4/mTOR signaling. In Cell death discovery, 8, 398. doi:10.1038/s41420-022-01170-7. https://pubmed.ncbi.nlm.nih.gov/36163180/
3. Koenig, Mary Kay, Bonnen, Penelope E. 2018. Metabolomics Profile in ABAT Deficiency Pre- and Post-treatment. In JIMD reports, 43, 13-17. doi:10.1007/8904_2018_94. https://pubmed.ncbi.nlm.nih.gov/29480352/
4. Lu, Jun, Chen, Zhan, Zhao, Hu, Chen, Shushang, Shao, Jichun. 2020. ABAT and ALDH6A1, regulated by transcription factor HNF4A, suppress tumorigenic capability in clear cell renal cell carcinoma. In Journal of translational medicine, 18, 101. doi:10.1186/s12967-020-02268-1. https://pubmed.ncbi.nlm.nih.gov/32093682/
5. Feng, Yan, Wei, Zi-Han, Liu, Chao, Zhang, Chu-Chu, Deng, Yan-Chun. 2022. Genetic variations in GABA metabolism and epilepsy. In Seizure, 101, 22-29. doi:10.1016/j.seizure.2022.07.007. https://pubmed.ncbi.nlm.nih.gov/35850019/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen