C57BL/6JCya-Slc4a11em1flox/Cya
Common Name:
Slc4a11-flox
Product ID:
S-CKO-09786
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc4a11-flox
Strain ID
CKOCMP-269356-Slc4a11-B6J-VA
Gene Name
Product ID
S-CKO-09786
Gene Alias
BTR1; NaBC1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc4a11em1flox/Cya mice (Catalog S-CKO-09786) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099362
NCBI RefSeq
NM_001081162
Target Region
Exon 4~8
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Slc4a11, a member of the bicarbonate transporter family, is an electrogenic H+ transporter activated by NH3 and alkaline pH [1]. Although it doesn't transport bicarbonate, it shares properties with other SLC4 family members. It plays a role in multiple biological processes, with its expression induced by oxidative stress via the transcription factor NRF2.
In Slc4a11 knockout (KO) mice, phenotypes such as corneal endothelial dystrophy and hearing deficits are observed, mirroring the conditions in humans with SLC4A11 mutations [1,2]. The inducible Slc4a11 KO mouse model shows that loss of Slc4a11 leads to corneal edema through perturbation of the corneal endothelial pump, accompanied by mitochondrial superoxide-induced Src kinase activation, disruption of tight junctions, and changes in lactate transporters and Na-K ATPase activity [4,5]. In hepatocellular carcinoma, SLC4A11 mediates ammonia import, promoting cancer stemness [3].
In conclusion, Slc4a11 is crucial for maintaining normal physiological functions, especially in the corneal endothelium, inner ear, and metabolism-related processes. The Slc4a11 KO and inducible KO mouse models have been instrumental in revealing its role in diseases like corneal endothelial dystrophy and in understanding its contribution to cancer stemness in hepatocellular carcinoma.
References:
1. Bonanno, Joseph A, Shyam, Raji, Choi, Moonjung, Ogando, Diego G. 2022. The H+ Transporter SLC4A11: Roles in Metabolism, Oxidative Stress and Mitochondrial Uncoupling. In Cells, 11, . doi:10.3390/cells11020197. https://pubmed.ncbi.nlm.nih.gov/35053313/
2. Patel, Sangita P, Parker, Mark D. 2015. SLC4A11 and the Pathophysiology of Congenital Hereditary Endothelial Dystrophy. In BioMed research international, 2015, 475392. doi:10.1155/2015/475392. https://pubmed.ncbi.nlm.nih.gov/26451371/
3. Elaimy, Ameer L, El-Derany, Marwa O, James, Jadyn, Shah, Yatrik M, Green, Michael D. 2024. SLC4A11 mediates ammonia import and promotes cancer stemness in hepatocellular carcinoma. In JCI insight, 9, . doi:10.1172/jci.insight.184826. https://pubmed.ncbi.nlm.nih.gov/39287988/
4. Ogando, Diego G, Shyam, Rajalekshmy, Kim, Edward T, Liu, Chia-Yang, Bonanno, Joseph A. . Inducible Slc4a11 Knockout Triggers Corneal Edema Through Perturbation of Corneal Endothelial Pump. In Investigative ophthalmology & visual science, 62, 28. doi:10.1167/iovs.62.7.28. https://pubmed.ncbi.nlm.nih.gov/34190974/
5. Ogando, Diego G, Kim, Edward T, Li, Shimin, Bonanno, Joseph A. 2023. Corneal Edema in Inducible Slc4a11 Knockout Is Initiated by Mitochondrial Superoxide Induced Src Kinase Activation. In Cells, 12, . doi:10.3390/cells12111528. https://pubmed.ncbi.nlm.nih.gov/37296649/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen