C57BL/6JCya-G3bp1em1flox/Cya
Common Name:
G3bp1-flox
Product ID:
S-CKO-09896
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
G3bp1-flox
Strain ID
CKOCMP-27041-G3bp1-B6J-VA
Gene Name
Product ID
S-CKO-09896
Gene Alias
B430204O07; G3bp; mKIAA4115
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-G3bp1em1flox/Cya mice (Catalog S-CKO-09896) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018727
NCBI RefSeq
NM_013716
Target Region
Exon 3~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
G3bp1, also known as Ras-GTPase-activating protein binding protein 1, is a multifunctional binding protein involved in diverse biological functions such as cell proliferation, metastasis, apoptosis, differentiation, and RNA metabolism [2]. It is a central node in the ribonucleoprotein (RNP) granule-related network, especially in the assembly of stress granules (SGs) through liquid-liquid phase separation (LLPS) triggered by a rise in intracellular free RNA concentrations [1]. It also participates in pathways like DNA sensing via enhancing DNA binding of cGAS [3].
In disease-related studies, G3bp1 has been shown to have context-specific roles. In various cancers, it acts as a cancer-promoting factor, facilitating cell proliferation, invasion, and metastasis [2]. In the context of viral infections, it can interact with viral proteins, either inhibiting viral replication as an antiviral factor or being hijacked by viruses to promote their proliferation [2]. In the case of sodium arsenite-induced ferroptosis, G3bp1 is indispensable through a stress-granule-independent mechanism, stabilizing IRP2 by suppressing FBXL5 mRNA translation, leading to elevated cellular labile free iron and subsequent ferroptotic cell death [5]. In bladder cancer, G3bp1, along with SLU7, promotes immune evasion by down-regulating MHC-I via PI3K/Akt activation [4].
In conclusion, G3bp1 is a key player in multiple biological functions and disease-related processes. Its study, especially through gene knockout or conditional knockout mouse models, has revealed its significance in areas such as cancer, viral infections, and ferroptosis-related kidney injury. Understanding G3bp1 provides insights into the underlying molecular mechanisms of these diseases, potentially paving the way for novel therapeutic strategies.
References:
1. Yang, Peiguo, Mathieu, Cécile, Kolaitis, Regina-Maria, Kim, Hong Joo, Taylor, J Paul. . G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. In Cell, 181, 325-345.e28. doi:10.1016/j.cell.2020.03.046. https://pubmed.ncbi.nlm.nih.gov/32302571/
2. Ge, Yidong, Jin, Jiabei, Li, Jinyun, Ye, Meng, Jin, Xiaofeng. 2022. The roles of G3BP1 in human diseases (review). In Gene, 821, 146294. doi:10.1016/j.gene.2022.146294. https://pubmed.ncbi.nlm.nih.gov/35176431/
3. Liu, Zhao-Shan, Cai, Hong, Xue, Wen, Zhang, Xue-Min, Li, Tao. 2018. G3BP1 promotes DNA binding and activation of cGAS. In Nature immunology, 20, 18-28. doi:10.1038/s41590-018-0262-4. https://pubmed.ncbi.nlm.nih.gov/30510222/
4. Zheng, Xianchong, Chen, Jiawei, Deng, Minhua, Kang, Tiebang, Liu, Zhuowei. 2023. G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2305922. doi:10.1002/advs.202305922. https://pubmed.ncbi.nlm.nih.gov/38084438/
5. Liu, Qian, Wang, Fengli, Chen, Yingxian, Cui, Hengkang, Wu, Hao. 2023. A regulatory module comprising G3BP1-FBXL5-IRP2 axis determines sodium arsenite-induced ferroptosis. In Journal of hazardous materials, 465, 133038. doi:10.1016/j.jhazmat.2023.133038. https://pubmed.ncbi.nlm.nih.gov/38118197/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen