C57BL/6NCya-Asnsem1flox/Cya
Common Name:
Asns-flox
Product ID:
S-CKO-09902
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Asns-flox
Strain ID
CKOCMP-27053-Asns-B6N-VA
Gene Name
Product ID
S-CKO-09902
Gene Alias
--
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Asnsem1flox/Cya mice (Catalog S-CKO-09902) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031766
NCBI RefSeq
NM_012055
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Asns, also known as asparagine synthetase (glutamine-hydrolyzing), is an enzyme that catalyzes the biosynthesis of asparagine, a non-essential amino acid. It is involved in multiple metabolic pathways, such as amino acid metabolism, and plays a crucial role in maintaining glutamine homeostasis [3]. Its function is essential for various biological processes, including cell differentiation and the response to metabolic stressors. Genetic models, like gene knockout mouse models, can be used to study its role in these processes.
In CD8+ T cells, the expression dynamics of Asns are critical for cell differentiation. Its expression peaks for effector T cells and decays during memory formation. Disrupting these dynamics by overexpressing Asns promotes an effector phenotype and enhances the anti-tumor response of adoptively transferred CD8+ T cells in a mouse melanoma model [1]. In osteosarcoma, NAT10 enhances the mRNA stability of ATF4 through ac4C modification, and ATF4 induces the transcription of Asns, which facilitates tumor progression. Inhibiting NAT10 suppresses osteosarcoma progression in vivo [2]. In clear cell renal cell carcinoma (ccRCC), Asns expression is increased, associated with advanced clinicopathological characteristics and T lymphocyte infiltration, and serves as an independent prognostic factor [4]. In acute liver injury, hepatocyte-specific Asns deletion in mice makes them more prone to pericentral liver damage after toxin exposure, and this can be reverted by intravenous administration of asparagine [5]. In pancreatic ductal adenocarcinoma (PDAC), inhibiting Asns by a glutamine antagonist reduces asparagine production and blocks tumor growth, and PDAC cells upregulate Asns as a metabolic adaptation [6].
In conclusion, Asns plays essential roles in cell differentiation, metabolism, and the response to stress in various biological systems. Studies using gene knockout or conditional knockout mouse models have revealed its significance in cancer, including melanoma, osteosarcoma, ccRCC, and PDAC, as well as in acute liver injury. Understanding Asns function provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Fernández-García, Juan, Franco, Fabien, Parik, Sweta, Ho, Ping-Chih, Fendt, Sarah-Maria. . CD8+ T cell metabolic rewiring defined by scRNA-seq identifies a critical role of ASNS expression dynamics in T cell differentiation. In Cell reports, 41, 111639. doi:10.1016/j.celrep.2022.111639. https://pubmed.ncbi.nlm.nih.gov/36384124/
2. Zou, Yutong, Guo, Siyao, Wen, Lili, Shen, Jingnan, Xie, Xianbiao. . Targeting NAT10 inhibits osteosarcoma progression via ATF4/ASNS-mediated asparagine biosynthesis. In Cell reports. Medicine, 5, 101728. doi:10.1016/j.xcrm.2024.101728. https://pubmed.ncbi.nlm.nih.gov/39293390/
3. Zhang, Shanshan, Feng, Han-Chao, Liu, Ji-Long. . ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae. In G3 (Bethesda, Md.), 11, . doi:10.1093/g3journal/jkaa060. https://pubmed.ncbi.nlm.nih.gov/33561249/
4. Gan, Xinqiang, Liu, Ruiji, Cheng, Hong, Feng, Ninghan, Chen, Ming. 2022. ASNS can predict the poor prognosis of clear cell renal cell carcinoma. In Frontiers in oncology, 12, 882888. doi:10.3389/fonc.2022.882888. https://pubmed.ncbi.nlm.nih.gov/36052245/
5. Sun, Yu, Demagny, Hadrien, Faure, Adrien, Perino, Alessia, Schoonjans, Kristina. 2023. Asparagine protects pericentral hepatocytes during acute liver injury. In The Journal of clinical investigation, 133, . doi:10.1172/JCI163508. https://pubmed.ncbi.nlm.nih.gov/36719750/
6. Recouvreux, Maria Victoria, Grenier, Shea F, Zhang, Yijuan, Tiriac, Hervé, Commisso, Cosimo. 2023. Glutamine mimicry suppresses tumor progression through asparagine metabolism in pancreatic ductal adenocarcinoma. In Nature cancer, 5, 100-113. doi:10.1038/s43018-023-00649-1. https://pubmed.ncbi.nlm.nih.gov/37814011/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen