C57BL/6JCya-Srsf12em1flox/Cya
Common Name:
Srsf12-flox
Product ID:
S-CKO-09951
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Srsf12-flox
Strain ID
CKOCMP-272009-Srsf12-B6J-VA
Gene Name
Product ID
S-CKO-09951
Gene Alias
B830039L16; Sfrs13b; Srrp; Srsf13b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srsf12em1flox/Cya mice (Catalog S-CKO-09951) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000067864
NCBI RefSeq
NM_177774
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Srsf12, a member of the serine-arginine rich splicing factor (SRSF) family, is involved in regulating RNA splicing processes [3,4,5]. It has been associated with various biological functions and pathways, and its dysregulation may impact different biological processes and disease states [1-3,7]. Genetic models, such as gene knockout or conditional knockout mouse models, can be valuable in further exploring its functions.
In a study on pancreatic cancer, Srsf12 knockout in Kras-mutated mice (KrasG12DSrsf12fl/fl and Srsf12fl/flPdx1cre mice) led to accelerated Kras-driven pancreatic tumorigenesis and enhanced tumor innervation. Mechanistically, SRSF12 inhibits neurite outgrowth by generating a LAMA3 splice isoform lacking the fourth and fifth LG (G45) domains, and G45 promotes tumor innervation by activating ITGB1 and FAK in neurons [2]. In acute myeloid leukemia (AML), SRSF12 is highly expressed and is an independent predictor of favorable prognosis. Enrichment analysis showed its involvement in pathways like PI3K-AKT and Jak-STAT, and its expression is correlated with immune cell infiltration [1].
In conclusion, Srsf12 plays crucial roles in regulating RNA splicing and is involved in important biological processes. Its functions have been revealed through in vivo studies, especially in the context of pancreatic cancer and AML. The use of KO/CKO mouse models has provided valuable insights into its role in tumorigenesis and immune-related processes in these disease areas.
References：
1. Zhang, MengMeng, Tan, YuanYuan, Xie, ZhiBin, Wang, Meng, Li, JiaJia. 2024. SRSF12 is a prognostic biomarker involved in immune infiltration in acute myeloid leukemia. In Hematology (Amsterdam, Netherlands), 29, 2420301. doi:10.1080/16078454.2024.2420301. https://pubmed.ncbi.nlm.nih.gov/39463061/
2. Yang, Min-Wei, Jia, Qin-Yuan, Xu, Da-Peng, Sun, Yong-Wei, Liu, Wei. 2025. SRSF12 deficiency enhances tumor innervation and accelerates pancreatic tumorigenesis. In Cancer letters, 616, 217563. doi:10.1016/j.canlet.2025.217563. https://pubmed.ncbi.nlm.nih.gov/39986371/
3. Shkreta, Lulzim, Delannoy, Aurélie, Toutant, Johanne, Chabot, Benoit. 2024. Regulatory interplay between SR proteins governs CLK1 kinase splice variants production. In RNA (New York, N.Y.), 30, 1596-1607. doi:10.1261/rna.080107.124. https://pubmed.ncbi.nlm.nih.gov/39251328/
4. Sharma, Sakshi, Mittal, Manasi, Shukla, Akanksha, Dinand, Veronique, Saluja, Daman. 2024. Exploring serine-arginine rich splicing factors: potential predictive markers for dysregulation in oral cancer. In BMC cancer, 24, 1094. doi:10.1186/s12885-024-12750-4. https://pubmed.ncbi.nlm.nih.gov/39227899/
5. More, Dhanashree Anil, Kumar, Arun. 2020. SRSF3: Newly discovered functions and roles in human health and diseases. In European journal of cell biology, 99, 151099. doi:10.1016/j.ejcb.2020.151099. https://pubmed.ncbi.nlm.nih.gov/32800280/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen