C57BL/6JCya-Prmt5em1flox/Cya
Common Name:
Prmt5-flox
Product ID:
S-CKO-10015
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prmt5-flox
Strain ID
CKOCMP-27374-Prmt5-B6J-VA
Gene Name
Product ID
S-CKO-10015
Gene Alias
Jbp1; Skb1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prmt5em1flox/Cya mice (Catalog S-CKO-10015) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023873
NCBI RefSeq
NM_013768
Target Region
Exon 2~6
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Prmt5, also known as Hsl7, Jbp1, Skb1, Capsuleen, or Dart5, is a predominant type II protein arginine methyltransferase. It catalyzes the mono-and symmetrical dimethylation of a wide range of histone and non-histone substrates, playing critical roles in numerous regulatory pathways, including those related to genome organization, transcription, cell cycle, and spliceosome assembly [3]. It is also involved in many normal cellular processes and is associated with cancer-related signaling pathways [1,2].
In high-risk neuroblastoma xenograft tumor models, pharmacologic or genetic inhibition of Prmt5 abolishes AKT1 arginine 15 methylation, preventing AKT1 translocation and subsequent activation, which in turn attenuates primary tumor growth and blocks metastasis [4]. In MTAP/CDKN2A-deleted cancers, depletion of Prmt5 impairs the viability of MTAP-deficient cancer cells as MTAP-deleted cells accumulate methylthioadenosine (MTA) that inhibits Prmt5 methyltransferase activity [5]. In triple-negative breast cancer, PRMT5 promotes ferroptosis resistance and impairs immunotherapy efficacy, and its inhibitors can potentiate immunotherapy [6]. In melanoma, reducing Prmt5 activity antagonizes melanoma growth in immunocompetent mice by affecting the cGAS/STING and NLRC5 pathways, and combination of Prmt5 inhibition with immune checkpoint therapy enhances therapeutic efficacy [7]. In colorectal cancer, Prmt5 methylating SMAD4 activates TGF-β signaling and promotes metastasis [8].
In conclusion, Prmt5 is essential for various cellular processes through its methylation functions. Studies using genetic inhibition or depletion models in different cancer types, such as neuroblastoma, MTAP/CDKN2A-deleted cancers, triple-negative breast cancer, melanoma, and colorectal cancer, have revealed its significance in tumor growth, metastasis, and response to immunotherapy. These findings suggest that Prmt5 could be a potential therapeutic target in these cancer conditions.
References:
1. Kim, Hyungsoo, Ronai, Ze'ev A. 2020. PRMT5 function and targeting in cancer. In Cell stress, 4, 199-215. doi:10.15698/cst2020.08.228. https://pubmed.ncbi.nlm.nih.gov/32743345/
2. Zheng, Jiahong, Li, Bang, Wu, Yingqi, Wu, Xiaoshuang, Wang, Yuanxiang. 2023. Targeting Arginine Methyltransferase PRMT5 for Cancer Therapy: Updated Progress and Novel Strategies. In Journal of medicinal chemistry, 66, 8407-8427. doi:10.1021/acs.jmedchem.3c00250. https://pubmed.ncbi.nlm.nih.gov/37366223/
3. Stopa, Nicole, Krebs, Jocelyn E, Shechter, David. 2015. The PRMT5 arginine methyltransferase: many roles in development, cancer and beyond. In Cellular and molecular life sciences : CMLS, 72, 2041-59. doi:10.1007/s00018-015-1847-9. https://pubmed.ncbi.nlm.nih.gov/25662273/
4. Huang, Lei, Zhang, Xiao-Ou, Rozen, Esteban J, Lee, Mary M, Wu, Qiong. 2022. PRMT5 activates AKT via methylation to promote tumor metastasis. In Nature communications, 13, 3955. doi:10.1038/s41467-022-31645-1. https://pubmed.ncbi.nlm.nih.gov/35803962/
5. Mavrakis, Konstantinos J, McDonald, E Robert, Schlabach, Michael R, Stegmeier, Frank, Sellers, William R. 2016. Disordered methionine metabolism in MTAP/CDKN2A-deleted cancers leads to dependence on PRMT5. In Science (New York, N.Y.), 351, 1208-13. doi:10.1126/science.aad5944. https://pubmed.ncbi.nlm.nih.gov/26912361/
6. Wang, Zhe, Li, Ruolei, Hou, Niuniu, Ling, Rui, Zhang, Jian. . PRMT5 reduces immunotherapy efficacy in triple-negative breast cancer by methylating KEAP1 and inhibiting ferroptosis. In Journal for immunotherapy of cancer, 11, . doi:10.1136/jitc-2023-006890. https://pubmed.ncbi.nlm.nih.gov/37380368/
7. Kim, Hyungsoo, Kim, Heejung, Feng, Yongmei, Tocci, Stefania, Ronai, Ze'ev A. . PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity. In Science translational medicine, 12, . doi:10.1126/scitranslmed.aaz5683. https://pubmed.ncbi.nlm.nih.gov/32641491/
8. Liu, Anyi, Yu, Chengxin, Qiu, Cheng, Hu, Junbo, Wang, Guihua. 2023. PRMT5 methylating SMAD4 activates TGF-β signaling and promotes colorectal cancer metastasis. In Oncogene, 42, 1572-1584. doi:10.1038/s41388-023-02674-x. https://pubmed.ncbi.nlm.nih.gov/36991117/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen