C57BL/6JCya-Shroom3em1flox/Cya
Common Name:
Shroom3-flox
Product ID:
S-CKO-10049
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Shroom3-flox
Strain ID
CKOCMP-27428-Shroom3-B6J-VA
Gene Name
Product ID
S-CKO-10049
Gene Alias
D5Ertd287e; Shrm; Shrm3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Shroom3em1flox/Cya mice (Catalog S-CKO-10049) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113055
NCBI RefSeq
NM_015756
Target Region
Exon 5
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Shroom3, encoding an actin-associated protein, is the sole member of the SHROOM protein group regulating actin dynamics via apical constriction and apicobasal cell elongation. It is involved in multiple biological processes, and its function is crucial in development and disease-related pathways [1].
In kidney-related studies, Shroom3-deficient mouse models have provided significant insights. Shroom3Gt/+ mice with 22-minute ischemic injury showed increased mortality, worsened kidney function, and disrupted epithelial redifferentiation, Rho-kinase/myosin signaling, and apical F-actin organization, indicating its importance in epithelial repair and redifferentiation after AKI and its link to CKD [2]. Shroom3 knockdown in mice led to albuminuria and podocyte foot process effacement, and in vitro, it caused defective lamellipodia formation in podocytes, disrupting the slit diaphragm, suggesting its role in maintaining podocyte structure and kidney function [4].
In optic cup development, Shroom3-deficient mouse embryos had coloboma phenotypes due to increased apical cell areas, disrupted optic fissure alignment, and failure to re-establish apical-basal polarity, highlighting its role in epithelial fusion events [3].
In cardiac development, Shroom3gt/gt mice exhibited a spectrum of congenital heart defects, demonstrating that SHROOM3 functions as an actomyosin cytoskeleton effector downstream of the planar cell polarity signaling pathway [5].
In conclusion, Shroom3 is essential for various biological processes such as epithelial morphogenesis, kidney function maintenance, optic cup development, and cardiac development. The study of Shroom3 KO/CKO mouse models has significantly contributed to understanding its roles in kidney diseases, ocular development, and congenital heart diseases, providing valuable insights into the underlying molecular mechanisms and potential therapeutic targets.
References:
1. Paul, Amy, Lawlor, Allison, Cunanan, Kristina, Lanktree, Matthew B, Bridgewater, Darren. 2023. The Good and the Bad of SHROOM3 in Kidney Development and Disease: A Narrative Review. In Canadian journal of kidney health and disease, 10, 20543581231212038. doi:10.1177/20543581231212038. https://pubmed.ncbi.nlm.nih.gov/38107159/
2. Li, Aihua, Cunanan, Joanna, Khalili, Hadiseh, Drysdale, Thomas, Bridgewater, Darren. 2021. Shroom3, a Gene Associated with CKD, Modulates Epithelial Recovery after AKI. In Kidney360, 3, 51-62. doi:10.34067/KID.0003802021. https://pubmed.ncbi.nlm.nih.gov/35368578/
3. Herstine, Jessica A, Mensh, Jordyn, Coffman, Electra, Bridgewater, Darren, Plageman, Timothy F. 2025. Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion. In Developmental biology, 522, 91-105. doi:10.1016/j.ydbio.2025.03.008. https://pubmed.ncbi.nlm.nih.gov/40113025/
4. Matsuura, Ryo, Hiraishi, Atsuko, Holzman, Lawrence B, Nangaku, Masaomi, Noiri, Eisei. 2020. SHROOM3, the gene associated with chronic kidney disease, affects the podocyte structure. In Scientific reports, 10, 21103. doi:10.1038/s41598-020-77952-9. https://pubmed.ncbi.nlm.nih.gov/33273487/
5. Durbin, Matthew D, O'Kane, James, Lorentz, Samuel, Firulli, Anthony B, Ware, Stephanie M. 2020. SHROOM3 is downstream of the planar cell polarity pathway and loss-of-function results in congenital heart defects. In Developmental biology, 464, 124-136. doi:10.1016/j.ydbio.2020.05.013. https://pubmed.ncbi.nlm.nih.gov/32511952/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen