C57BL/6JCya-Timd4em1flox/Cya
Common Name:
Timd4-flox
Product ID:
S-CKO-10059
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Timd4-flox
Strain ID
CKOCMP-276891-Timd4-B6J-VA
Gene Name
Product ID
S-CKO-10059
Gene Alias
B430010N18Rik; TIM-4; Tim4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Timd4em1flox/Cya mice (Catalog S-CKO-10059) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000068877
NCBI RefSeq
NM_178759
Target Region
Exon 2~3
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Timd4, also known as T cell immunoglobulin and mucin domain containing 4, is significantly involved in macrophage-related functions and various biological processes. It serves as an important receptor in efferocytosis, the clearance of apoptotic cells, which is crucial for tissue homeostasis and prevention of autoimmunity. Additionally, it may be associated with pathways like Wnt/β-catenin in certain cell types [1,3].
In mouse models, Timd4 deficiency leads to reduced CD301b+ macrophages and aggravated periodontitis bone loss, indicating its role in maintaining the osteoimmunology microenvironment in periodontitis [2]. In skin wound repair in mice, inhibition of Timd4 decreases efferocytosis and abrogates wound repair, highlighting its importance in the process of tissue repair [5]. In the context of cancer, Tim-4+ cavity-resident macrophages can impair anti-tumor CD8+ T cell immunity, and Tim-4 blockade can enhance anti-tumor efficacy in mouse models of immunotherapy [4].
In conclusion, Timd4 plays essential roles in macrophage-mediated processes such as efferocytosis, tissue repair, and immune regulation in various disease conditions. Studies using gene-knockout mouse models have provided valuable insights into its functions in periodontitis, skin wound repair, and cancer immunotherapy, suggesting that Timd4 could be a potential therapeutic target for these diseases.
References:
1. Li, Yi, Zhang, Pei-Yi, Yang, Zhao-Wen, Ma, Fang, Li, Fa-Xin. 2020. TIMD4 exhibits regulatory capability on the proliferation and apoptosis of diffuse large B-cell lymphoma cells via the Wnt/β-catenin pathway. In The journal of gene medicine, 22, e3186. doi:10.1002/jgm.3186. https://pubmed.ncbi.nlm.nih.gov/32187802/
2. Wang, Ziming, Zeng, Hao, Wang, Can, Zhao, Qin, Zhang, Yufeng. 2024. Tim4 deficiency reduces CD301b+ macrophage and aggravates periodontitis bone loss. In International journal of oral science, 16, 20. doi:10.1038/s41368-023-00270-z. https://pubmed.ncbi.nlm.nih.gov/38418808/
3. Saavedra, Pedro H V, Trzeciak, Alissa J, Lipshutz, Allie, Rudensky, Alexander Y, Perry, Justin S A. 2024. Broad-spectrum antibiotics disrupt homeostatic efferocytosis. In Nature metabolism, 6, 1682-1694. doi:10.1038/s42255-024-01107-7. https://pubmed.ncbi.nlm.nih.gov/39122784/
4. Chow, Andrew, Schad, Sara, Green, Michael D, Rudin, Charles M, Merghoub, Taha. 2021. Tim-4+ cavity-resident macrophages impair anti-tumor CD8+ T cell immunity. In Cancer cell, 39, 973-988.e9. doi:10.1016/j.ccell.2021.05.006. https://pubmed.ncbi.nlm.nih.gov/34115989/
5. Justynski, Olivia, Bridges, Kate, Krause, Will, Miller-Jensen, Kathryn, Horsley, Valerie. 2023. Apoptosis recognition receptors regulate skin tissue repair in mice. In eLife, 12, . doi:10.7554/eLife.86269. https://pubmed.ncbi.nlm.nih.gov/38127424/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen