C57BL/6JCya-Vkorc1em1flox/Cya
Common Name:
Vkorc1-flox
Product ID:
S-CKO-10106
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Vkorc1-flox
Strain ID
CKOCMP-27973-Vkorc1-B6J-VA
Gene Name
Product ID
S-CKO-10106
Gene Alias
D7Wsu86e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vkorc1em1flox/Cya mice (Catalog S-CKO-10106) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033074
NCBI RefSeq
NM_178600
Target Region
Exon 2~3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
VKORC1, short for Vitamin K epoxide reductase complex subunit 1, is a key enzyme in the vitamin K cycle. It is responsible for reducing vitamin K epoxide back to its active form, which is essential for the γ -carboxylation of vitamin K-dependent proteins involved in blood coagulation [2,4]. This process is crucial for maintaining normal blood clotting function, and VKORC1 is also the molecular target of coumarins, a type of anticoagulant drug [2].
Genetic studies have shown significant associations related to VKORC1. Polymorphisms in VKORC1 play a major role in warfarin dose variation, as different ethnic groups have distinct VKORC1 genotypes, influencing warfarin requirements [1]. Mutations in VKORC1 can lead to a coumarin-resistant phenotype, and a specific SNP (rs9923231) in its promoter region is a major pharmacodynamic determinant of coumarin dose [2]. Additionally, in rodents, SNPs in vkorc1 are associated with resistance to anticoagulant rodenticides [3,6]. In humans, VKORC1 rs9923231 polymorphism may increase the risk of osteoporosis [5].
In conclusion, VKORC1 is vital for the vitamin K cycle and blood coagulation. Genetic models have revealed its role in drug response, such as warfarin dosing, and in disease conditions like osteoporosis and rodenticide resistance in rodents. Understanding VKORC1 through these models provides valuable insights into anticoagulant therapy and bone-related diseases.
References:
1. Gaikwad, Tejasvita, Ghosh, Kanjaksha, Shetty, Shrimati. 2014. VKORC1 and CYP2C9 genotype distribution in Asian countries. In Thrombosis research, 134, 537-44. doi:10.1016/j.thromres.2014.05.028. https://pubmed.ncbi.nlm.nih.gov/24908449/
2. Oldenburg, J, Watzka, M, Rost, S, Müller, C R. . VKORC1: molecular target of coumarins. In Journal of thrombosis and haemostasis : JTH, 5 Suppl 1, 1-6. doi:. https://pubmed.ncbi.nlm.nih.gov/17635701/
3. Bermejo-Nogales, Azucena, Rodríguez Martín, José A, Coll, Julio, Navas, José M. 2022. VKORC1 single nucleotide polymorphisms in rodents in Spain. In Chemosphere, 308, 136021. doi:10.1016/j.chemosphere.2022.136021. https://pubmed.ncbi.nlm.nih.gov/35970209/
4. Garcia, Andrea A, Reitsma, Pieter H. . VKORC1 and the vitamin K cycle. In Vitamins and hormones, 78, 23-33. doi:10.1016/S0083-6729(07)00002-7. https://pubmed.ncbi.nlm.nih.gov/18374188/
5. He, Jin, Xie, Hua, Yan, Chengyuan, Xu, Zhonghua, Zhang, Xing. 2020. Genetic Variation in VKORC1 and Risk for Osteoporosis. In Archives of medical research, 52, 211-216. doi:10.1016/j.arcmed.2020.10.008. https://pubmed.ncbi.nlm.nih.gov/33234259/
6. Yiğit, Nuri, Duman, Mustafa T, Çetintürk, Derya, Çolak, Ercüment, Çolak, Reyhan. 2023. Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats. In PeerJ, 11, e15055. doi:10.7717/peerj.15055. https://pubmed.ncbi.nlm.nih.gov/37151288/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen