C57BL/6JCya-Kif19aem1flox/Cya
Common Name:
Kif19a-flox
Product ID:
S-CKO-10160
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kif19a-flox
Strain ID
CKOCMP-286942-Kif19a-B6J-VA
Gene Name
Product ID
S-CKO-10160
Gene Alias
Kif19
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kif19aem1flox/Cya mice (Catalog S-CKO-10160) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000084368
NCBI RefSeq
NM_001102615
Target Region
Exon 9~10
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Kif19a, a member of the kinesin superfamily, is a microtubule-depolymerizing kinesin crucial for ciliary length control [2,4]. It localizes to cilia tips and its function is integral to the proper generation of fluid flow by cilia, which is essential for maintaining homeostasis in the mammalian body [2]. The AC6-AMPK pathway has been identified as an important regulator of Kif19a, where AC6 inhibits the degradation of Kif19a by suppressing autophagy [1].
Kif19a knockout (KO) mice display hydrocephalus and female infertility phenotypes due to abnormally elongated cilia that can't generate proper fluid flow, demonstrating the importance of Kif19a in ciliary length-related physiological functions [2]. In epithelium-specific knockout mice of AC6, airway epithelial cells have longer cilia because of decreased Kif19a protein levels in the cilia, suggesting that the AC6-AMPK pathway is a crucial ciliary growth checkpoint [1]. Additionally, in C. elegans, mutation in the depolymerizing kinesin-8 KLP-13/KIF19A suppresses the ciliary defects caused by a non-degradable nekl-4(PESTΔ) mutant, indicating its role in ciliary stability regulation [3,5].
In conclusion, Kif19a is essential for ciliary length control and proper fluid flow generation in mammals. The use of KO and conditional knockout (CKO) mouse models has been instrumental in revealing its role in cilia-associated disorders such as hydrocephalus and female infertility. Understanding the regulation of Kif19a through pathways like AC6-AMPK provides potential therapeutic targets for ciliopathies.
References:
1. Arora, Kavisha, Lund, John R, Naren, Nevin A, Zingarelli, Basilia, Naren, Anjaparavanda P. 2020. AC6 regulates the microtubule-depolymerizing kinesin KIF19A to control ciliary length in mammals. In The Journal of biological chemistry, 295, 14250-14259. doi:10.1074/jbc.RA120.013703. https://pubmed.ncbi.nlm.nih.gov/32683324/
2. Niwa, Shinsuke, Nakajima, Kazuo, Miki, Harukata, Wang, Doudou, Hirokawa, Nobutaka. 2012. KIF19A is a microtubule-depolymerizing kinesin for ciliary length control. In Developmental cell, 23, 1167-75. doi:10.1016/j.devcel.2012.10.016. https://pubmed.ncbi.nlm.nih.gov/23168168/
3. Power, Kaiden M, Nguyen, Ken C, Silva, Andriele, Rongo, Christopher, Barr, Maureen M. 2024. NEKL-4 regulates microtubule stability and mitochondrial health in ciliated neurons. In The Journal of cell biology, 223, . doi:10.1083/jcb.202402006. https://pubmed.ncbi.nlm.nih.gov/38767515/
4. Niwa, Shinsuke. 2014. Kinesin superfamily proteins and the regulation of microtubule dynamics in morphogenesis. In Anatomical science international, 90, 1-6. doi:10.1007/s12565-014-0259-5. https://pubmed.ncbi.nlm.nih.gov/25347970/
5. Power, Kaiden M, Nguyen, Ken C, Silva, Andriele, Rongo, Christopher, Barr, Maureen M. 2024. NEKL-4 regulates microtubule stability and mitochondrial health in C. elegans ciliated neurons. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.02.14.580304. https://pubmed.ncbi.nlm.nih.gov/38405845/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen