C57BL/6JCya-Lmcd1em1flox/Cya
Common Name:
Lmcd1-flox
Product ID:
S-CKO-10233
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lmcd1-flox
Strain ID
CKOCMP-30937-Lmcd1-B6J-VA
Gene Name
Product ID
S-CKO-10233
Gene Alias
dyxin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lmcd1em1flox/Cya mice (Catalog S-CKO-10233) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032376
NCBI RefSeq
NM_144799
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Lmcd1, or LIM and cysteine rich domains 1, is a transcription factor involved in multiple biological processes. It is functionally engaged in protein-protein interactions with transcriptional co-repressor activity and is associated with pathways like the calcineurin-NFAT signaling cascade, which is crucial for recovery from acute kidney injury [1].
In renal fibrosis, silencing Lmcd1 in 7-day unilateral ureteral obstruction (7UUO) mouse models and TNF-α-stimulated HK-2 cell models alleviated tubulointerstitial inflammation, collagen deposition, and reduced profibrotic factors' expression. Mechanistically, Lmcd1 cooperates with NFATc1 to increase NLRP3 expression [2].
In a 21-day unilateral ureteral obstruction (21UUO) mouse model, Lmcd1 was associated with renal interstitial fibrosis, and knockdown of Lmcd1 in UUO mice mitigated renal histopathological changes, interstitial fibrosis, and cell apoptosis, blocking the activation of ERK signaling [3].
In testicular function, ablation of endogenous Lmcd1 expression in Sertoli cells caused lipid accumulation and senescence, and depletion of Lmcd1 in vivo impaired male fertility, as Lmcd1 facilitated the dephosphorylation and nuclear translocation of NFAT1 to promote phagocytosis of apoptotic germ cells [4].
In summary, Lmcd1 plays essential roles in processes such as renal function recovery, renal fibrosis, and testicular function. Gene-knockout and related in-vivo studies in mouse models have revealed its significance in these biological processes and potential as a therapeutic target for diseases like renal fibrosis and testicular dysfunction [2,3,4].
References:
1. Yalman, Nesil. . LMCD1 antisense RNA 1 is a newly identified long noncoding RNA. In Anti-cancer drugs, 33, 1-5. doi:10.1097/CAD.0000000000001124. https://pubmed.ncbi.nlm.nih.gov/34232945/
2. Bai, Yu, Tian, Mi, He, Ping, Lan, Jingsi, Zhang, Beiru. 2023. LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation. In International immunopharmacology, 121, 110362. doi:10.1016/j.intimp.2023.110362. https://pubmed.ncbi.nlm.nih.gov/37311356/
3. Yu, Rui, Tian, Mi, He, Ping, Zhang, Yongzhe, Zhang, Beiru. 2021. Suppression of LMCD1 ameliorates renal fibrosis by blocking the activation of ERK pathway. In Biochimica et biophysica acta. Molecular cell research, 1869, 119200. doi:10.1016/j.bbamcr.2021.119200. https://pubmed.ncbi.nlm.nih.gov/34968577/
4. Jin, Xiaohang, Zhang, Sheng, Ding, Tianbing, Zhang, Yuxing, Li, Wei. 2020. Testicular Lmcd1 regulates phagocytosis by Sertoli cells through modulation of NFAT1/Txlna signaling pathway. In Aging cell, 19, e13217. doi:10.1111/acel.13217. https://pubmed.ncbi.nlm.nih.gov/32840323/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen