C57BL/6JCya-Zfp182em1flox/Cya
Common Name:
Zfp182-flox
Product ID:
S-CKO-10302
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zfp182-flox
Strain ID
CKOCMP-319535-Zfp182-B6J-VA
Gene Name
Product ID
S-CKO-10302
Gene Alias
6330583I20Rik; Znf182
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp182em1flox/Cya mice (Catalog S-CKO-10302) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115333
NCBI RefSeq
NM_001013387
Target Region
Exon 3~4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Zfp182, a TFIIIA-type zinc finger protein, is involved in multiple abiotic stress responses in plants. It plays a role in pathways related to abscisic acid (ABA)-induced antioxidant defense, salt, cold and drought tolerances. Understanding its function is crucial for improving stress tolerance in crops [1,2,3].
In rice, ABA treatment induces Zfp182 expression, and it is required for ABA-induced antioxidant defense as transient gene expression and RNAi analysis in protoplasts showed its involvement in up-regulating superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities. Also, rice MAPKs, OsMPK1 and OsMPK5, are required for Zfp182 up-regulation in ABA signaling [1]. Overexpression of Zfp182 in transgenic rice significantly enhanced multiple abiotic stress tolerances, promoting the accumulation of compatible osmolytes and activating stress-related genes like OsP5CS, OsLEA3, OsDREB1A and OsDREB1B [2]. Expression of Zfp182 in transgenic tobacco and overexpression in rice increased plant tolerance to salt stress [3]. In wheat, mutations in NAC transcription factors affected the transcription pattern of Zfp182 under salt or drought stress [4]. In rice, a superior haplotype of Zfp182 was associated with higher single plant yield under drought stress [5].
In conclusion, Zfp182 is essential for plants' responses to various abiotic stresses, including those mediated by ABA, salt, cold and drought. Its study in plant models provides insights into stress-tolerance mechanisms, offering potential for improving crop resilience to environmental challenges.
References:
1. Zhang, Hong, Ni, Lan, Liu, Yanpei, Tan, Mingpu, Jiang, Mingyi. . The C2H2-type zinc finger protein ZFP182 is involved in abscisic acid-induced antioxidant defense in rice. In Journal of integrative plant biology, 54, 500-10. doi:10.1111/j.1744-7909.2012.01135.x. https://pubmed.ncbi.nlm.nih.gov/22693960/
2. Huang, Ji, Sun, Shujing, Xu, Dongqing, Tang, Haijuan, Zhang, Hongsheng. 2012. A TFIIIA-type zinc finger protein confers multiple abiotic stress tolerances in transgenic rice (Oryza sativa L.). In Plant molecular biology, 80, 337-50. doi:10.1007/s11103-012-9955-5. https://pubmed.ncbi.nlm.nih.gov/22930448/
3. Huang, Ji, Yang, Xia, Wang, Mei-Mei, Shen, Yin, Zhang, Hong-Sheng. 2007. A novel rice C2H2-type zinc finger protein lacking DLN-box/EAR-motif plays a role in salt tolerance. In Biochimica et biophysica acta, 1769, 220-7. doi:. https://pubmed.ncbi.nlm.nih.gov/17434609/
4. Wang, Dongzhi, Li, Yongpeng, Wang, Haojie, Liu, Xigang, Xiao, Jun. 2023. Boosting wheat functional genomics via an indexed EMS mutant library of KN9204. In Plant communications, 4, 100593. doi:10.1016/j.xplc.2023.100593. https://pubmed.ncbi.nlm.nih.gov/36945776/
5. Singh, Preeti, Sundaram, Krishna T, Vinukonda, Vishnu Prasanth, Singh, Vikas K, Sinha, Pallavi. 2024. Superior haplotypes of key drought-responsive genes reveal opportunities for the development of climate-resilient rice varieties. In Communications biology, 7, 89. doi:10.1038/s42003-024-05769-7. https://pubmed.ncbi.nlm.nih.gov/38216712/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen