C57BL/6JCya-Zdhhc17em1flox/Cya
Common Name:
Zdhhc17-flox
Product ID:
S-CKO-10398
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zdhhc17-flox
Strain ID
CKOCMP-320150-Zdhhc17-B6J-VA
Gene Name
Product ID
S-CKO-10398
Gene Alias
A230053P19Rik; D130071N24Rik; DHHC-17; Hip14
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zdhhc17em1flox/Cya mice (Catalog S-CKO-10398) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041723
NCBI RefSeq
NM_172554
Target Region
Exon 4~5
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Zdhhc17, a member of the zinc finger DHHC-domain containing (ZDHHC) family, is an S-acyltransferase crucial for S-palmitoylation, a post-translational modification. This process impacts protein localization, stability, and function, and is involved in various cellular pathways [1].
In high-fat diet-induced liver tumorigenesis, Zdhhc17 knockout mice helped reveal that Zdhhc17 palmitoylates AKT, promoting its activation, thus driving NASH and liver cancer development [2]. In polycystic ovary syndrome (PCOS), Zdhhc17 depletion in granulosa cells decreased HSP90α palmitoylation, hampering androgen to estrogen conversion, suggesting its role in PCOS pathophysiology [3]. In glioblastoma, Zdhhc17 knockout-related studies showed that palmitoylation mediated by Zdhhc17 preserves Oct4A from lysosome degradation, maintaining GSC tumorigenicity and stemness [4]. Also, in nervous system development, loss-of-function of Zdhhc17 in zebrafish led to motor dysfunction due to defective axonal outgrowth of spinal motor neurons [5].
In conclusion, Zdhhc17 plays essential roles in multiple biological processes and disease conditions. Through gene knockout models in different organisms, its functions in processes like liver tumorigenesis, PCOS, glioblastoma development, and nervous system development have been elucidated, highlighting its potential as a therapeutic target in related diseases.
References:
1. Zhang, Ying, Fan, Sisi, He, Lu, Li, Lanfang. 2024. The ZDHHC13/ZDHHC17 subfamily: From biological functions to therapeutic targets of diseases. In Pharmacological research, 209, 107418. doi:10.1016/j.phrs.2024.107418. https://pubmed.ncbi.nlm.nih.gov/39306022/
2. Bu, Lang, Zhang, Zhengkun, Chen, Jianwen, Kuang, Ming, Guo, Jianping. 2024. High-fat diet promotes liver tumorigenesis via palmitoylation and activation of AKT. In Gut, 73, 1156-1168. doi:10.1136/gutjnl-2023-330826. https://pubmed.ncbi.nlm.nih.gov/38191266/
3. Zhao, Shanmeizi, Ma, Rujun, Jueraitetibaike, Kadiliya, Yao, Bing, Guo, Zhigang. 2023. ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells. In Molecular and cellular endocrinology, 578, 112076. doi:10.1016/j.mce.2023.112076. https://pubmed.ncbi.nlm.nih.gov/37769867/
4. Chen, Xueran, Niu, Wanxiang, Fan, Xiaoqing, Yao, Xuebiao, Fang, Zhiyou. . Oct4A palmitoylation modulates tumorigenicity and stemness in human glioblastoma cells. In Neuro-oncology, 25, 82-96. doi:10.1093/neuonc/noac157. https://pubmed.ncbi.nlm.nih.gov/35727735/
5. Shi, Wei, Wang, Fen, Gao, Ming, Chen, Xueran, Hao, Aijun. 2015. ZDHHC17 promotes axon outgrowth by regulating TrkA-tubulin complex formation. In Molecular and cellular neurosciences, 68, 194-202. doi:10.1016/j.mcn.2015.07.005. https://pubmed.ncbi.nlm.nih.gov/26232532/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen