C57BL/6JCya-Slc39a9em1flox/Cya
Common Name
Slc39a9-flox
Product ID
S-CKO-10560
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-328133-Slc39a9-B6J-VA
Status
When using this mouse strain in a publication, please cite “Slc39a9-flox Mouse (Catalog S-CKO-10560) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Slc39a9-flox
Strain ID
CKOCMP-328133-Slc39a9-B6J-VA
Gene Name
Product ID
S-CKO-10560
Gene Alias
2010002A02Rik, 2610511I23Rik, 4833420E20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 12
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000085245
NCBI RefSeq
NM_026244
Target Region
Exon 2~3
Size of Effective Region
~4.8 kb
Overview of Gene Research
Slc39a9, also known as ZIP9, is a zinc transporter that has been identified as a membrane androgen receptor in various teleost and mammalian cell models [2]. It plays crucial roles in regulating zinc homeostasis, particularly in the secretory pathway [4]. It is involved in multiple biological processes, including egg activation, cell migration, and viral entry, and is associated with diseases like cancer [2,3,5]. Genetic models such as gene-knockout models have been instrumental in studying its functions.
In zebrafish, zip9-/- females showed reduced fecundity, embryo viability, and abnormal egg activation with depressed zinc release, indicating ZIP9's essential role in proper zinc modulation during egg activation [2]. In breast cancer cells, ZIP9 overexpression increased zinc levels and cell migration/invasion, while knockdown decreased these processes, suggesting its role in tumorigenic responses [3]. A genome-wide CRISPR/Cas9 screen identified SLC39A9 as a crucial entry factor for Ebola virus infection, with its genetic depletion reducing viral entry [1]. In periodontal ligament cells, knockdown of genes regulating SLC39A9 RNA m6A modification affected IL-6 expression, suggesting its involvement in the inflammatory response [6].
In conclusion, Slc39a9 is essential for zinc-related biological processes such as egg activation, cell migration, and immune-related responses. Gene-knockout models have revealed its significance in diseases like cancer and viral infections, providing valuable insights into potential therapeutic targets for these conditions.
References：
1. Gong, Mingli, Peng, Cheng, Yang, Chen, Shan, Chao, Ding, Qiang. 2024. Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection. In PLoS pathogens, 20, e1012444. doi:10.1371/journal.ppat.1012444. https://pubmed.ncbi.nlm.nih.gov/39173055/
2. Converse, Aubrey, Thomas, Peter. 2020. The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish. In Scientific reports, 10, 15673. doi:10.1038/s41598-020-72515-4. https://pubmed.ncbi.nlm.nih.gov/32973303/
3. Thomas, Peter, Pang, Yefei, Dong, Jing. 2023. Ligand-independent signaling and migration of breast cancer cells expressing membrane androgen receptor, ZIP9 (SLC39A9). In Molecular and cellular endocrinology, 578, 112060. doi:10.1016/j.mce.2023.112060. https://pubmed.ncbi.nlm.nih.gov/37660782/
4. Matsuura, Wataru, Yamazaki, Tomohiro, Yamaguchi-Iwai, Yuko, Andrews, Glen K, Kambe, Taiho. 2009. SLC39A9 (ZIP9) regulates zinc homeostasis in the secretory pathway: characterization of the ZIP subfamily I protein in vertebrate cells. In Bioscience, biotechnology, and biochemistry, 73, 1142-8. doi:. https://pubmed.ncbi.nlm.nih.gov/19420709/
5. Chen, Jinbo, Chou, Fuju, Yeh, Shuyuan, Zu, Xiongbing, Chang, Chawnshang. 2019. Androgen dihydrotestosterone (DHT) promotes the bladder cancer nuclear AR-negative cell invasion via a newly identified membrane androgen receptor (mAR-SLC39A9)-mediated Gαi protein/MAPK/MMP9 intracellular signaling. In Oncogene, 39, 574-586. doi:10.1038/s41388-019-0964-6. https://pubmed.ncbi.nlm.nih.gov/31506605/
6. Huang, Jing, Wang, Yining, Zhou, Yi. 2023. METTL3 and METTL14 regulate IL-6 expression via RNA m6A modification of zinc transporter SLC39A9 and DNA methylation of IL-6 in periodontal ligament cells. In Biochimica et biophysica acta. Molecular cell research, 1871, 119605. doi:10.1016/j.bbamcr.2023.119605. https://pubmed.ncbi.nlm.nih.gov/37821055/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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