C57BL/6JCya-Ubash3aem1flox/Cya
Common Name:
Ubash3a-flox
Product ID:
S-CKO-10589
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ubash3a-flox
Strain ID
CKOCMP-328795-Ubash3a-B6J-VA
Gene Name
Product ID
S-CKO-10589
Gene Alias
5830413C03Rik; C330001M22; Sts-2; TULA; TULA-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ubash3aem1flox/Cya mice (Catalog S-CKO-10589) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000236745
NCBI RefSeq
NM_177823
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Ubash3a, also known as Ubiquitin Associated and SH3 Domain Containing A, is a negative regulator of T cell function. It plays a crucial role in autoimmunity and is involved in pathways such as endoplasmic reticulum-associated protein degradation, cell motility, endocytosis, and endocytic recycling of membrane receptors [1]. It has been associated with multiple autoimmune diseases, and its study using genetic models can provide insights into its functions.
In NOD mice, Ubash3a-deficient mice showed more advanced insulitis and accelerated development of type 1 diabetes (T1D), with increased accumulation of β-cell autoreactive T cells in the spleen and pancreatic lymph node. Adoptive transfer of splenic T cells from Ubash3a-deficient mice into NOD.Rag1-/-mice promoted T1D development, indicating that Ubash3a deficiency in T cells is sufficient to drive T1D. Additionally, Ubash3a deficiency promoted salivary gland inflammation in female NOD mice [2].
In conclusion, Ubash3a is a key negative regulator of T cell function, with its deficiency promoting the development of type 1 diabetes and salivary gland inflammation. The study of Ubash3a using gene-knockout mouse models has significantly enhanced our understanding of its role in autoimmunity, particularly in T1D, providing potential targets for the treatment of these diseases.
References:
1. Ge, Yan, Paisie, Taylor K, Chen, Sixue, Concannon, Patrick. 2019. UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes. In Journal of immunology (Baltimore, Md. : 1950), 203, 2827-2836. doi:10.4049/jimmunol.1801338. https://pubmed.ncbi.nlm.nih.gov/31659016/
2. Chen, Yi-Guang, Ciecko, Ashley E, Khaja, Shamim, Geurts, Aron M, Lieberman, Scott M. 2020. UBASH3A deficiency accelerates type 1 diabetes development and enhances salivary gland inflammation in NOD mice. In Scientific reports, 10, 12019. doi:10.1038/s41598-020-68956-6. https://pubmed.ncbi.nlm.nih.gov/32694640/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen