C57BL/6JCya-Ubash3aem1flox/Cya
Common Name
Ubash3a-flox
Product ID
S-CKO-10589
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-328795-Ubash3a-B6J-VA
When using this mouse strain in a publication, please cite “Ubash3a-flox Mouse (Catalog S-CKO-10589) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ubash3a-flox
Strain ID
CKOCMP-328795-Ubash3a-B6J-VA
Gene Name
Product ID
S-CKO-10589
Gene Alias
TULA, Sts-2, TULA-1, C330001M22, 5830413C03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000236745
NCBI RefSeq
NM_177823
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Overview of Gene Research
Ubash3a, also known as Ubiquitin Associated and SH3 Domain Containing A, is a negative regulator of T cell function. It plays a crucial role in autoimmunity and is involved in pathways such as endoplasmic reticulum-associated protein degradation, cell motility, endocytosis, and endocytic recycling of membrane receptors [1]. It has been associated with multiple autoimmune diseases, and its study using genetic models can provide insights into its functions.
In NOD mice, Ubash3a-deficient mice showed more advanced insulitis and accelerated development of type 1 diabetes (T1D), with increased accumulation of β-cell autoreactive T cells in the spleen and pancreatic lymph node. Adoptive transfer of splenic T cells from Ubash3a-deficient mice into NOD.Rag1-/-mice promoted T1D development, indicating that Ubash3a deficiency in T cells is sufficient to drive T1D. Additionally, Ubash3a deficiency promoted salivary gland inflammation in female NOD mice [2].
In conclusion, Ubash3a is a key negative regulator of T cell function, with its deficiency promoting the development of type 1 diabetes and salivary gland inflammation. The study of Ubash3a using gene-knockout mouse models has significantly enhanced our understanding of its role in autoimmunity, particularly in T1D, providing potential targets for the treatment of these diseases.
References：
1. Ge, Yan, Paisie, Taylor K, Chen, Sixue, Concannon, Patrick. 2019. UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes. In Journal of immunology (Baltimore, Md. : 1950), 203, 2827-2836. doi:10.4049/jimmunol.1801338. https://pubmed.ncbi.nlm.nih.gov/31659016/
2. Chen, Yi-Guang, Ciecko, Ashley E, Khaja, Shamim, Geurts, Aron M, Lieberman, Scott M. 2020. UBASH3A deficiency accelerates type 1 diabetes development and enhances salivary gland inflammation in NOD mice. In Scientific reports, 10, 12019. doi:10.1038/s41598-020-68956-6. https://pubmed.ncbi.nlm.nih.gov/32694640/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.