C57BL/6JCya-Acsbg2em1flox/Cya
Common Name:
Acsbg2-flox
Product ID:
S-CKO-10592
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Acsbg2-flox
Strain ID
CKOCMP-328845-Acsbg2-B6J-VA
Gene Name
Product ID
S-CKO-10592
Gene Alias
Bgr
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acsbg2em1flox/Cya mice (Catalog S-CKO-10592) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043062
NCBI RefSeq
NM_001039114
Target Region
Exon 3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Acsbg2, a member of the acyl-CoA synthetase bubblegum family, is involved in activating fatty acids to their CoA derivatives, playing a role in fatty acid metabolism [3]. It is specifically expressed in the testis and brainstem in humans, mice, and rats [3]. In vertebrates, the Acsbg gene family, including Acsbg2, has an evolutionary history with gene expansion, retention, and loss events [4].
In chickens, miR-125b-5p can directly bind to the 3'UTR of Acsbg2, and miR-125b-5p overexpression represses Acsbg2 mRNA and protein expression. This interaction affects adipogenesis, as miR-125b-5p inhibits preadipocyte proliferation and promotes differentiation, at least partially by downregulating Acsbg2 [1]. In chicken studies, the G1257069A and T1247123C of the Acsbg2 gene were significantly associated with abdominal fat weight, indicating its role in fat deposition [6]. Also, in chicken liver, Acsbg2 was identified as a hub gene in abdominal fat weight-positively correlated modules, suggesting its importance in lipid metabolism [8].
In dairy goats, Acsbg2 was identified as a candidate gene related to milk production traits [5]. In Chinese Holstein population, a variant in Acsbg2 affected fat yield and protein percentage [7]. In early Alzheimer's disease, Acsbg2 was one of the core targets for lipid metabolism disorder [2].
In summary, Acsbg2 is crucial for fatty acid metabolism and is involved in various biological processes such as adipogenesis, fat deposition, milk production, and lipid metabolism in different species. Its study in these genetic models provides insights into its functions in normal physiological processes and disease-related lipid metabolism disorders, like in early Alzheimer's disease [1,2,5,6,7,8].
References:
1. Li, Guoxi, Chen, Yi, Jin, Wenjiao, Kang, Xiangtao, Tian, Yadong. 2021. Effects of miR-125b-5p on Preadipocyte Proliferation and Differentiation in Chicken. In Molecular biology reports, 48, 491-502. doi:10.1007/s11033-020-06080-4. https://pubmed.ncbi.nlm.nih.gov/33398680/
2. Wang, Linshuang, Qu, Fengxue, Yu, Xueyun, Han, Xuejie, Wei, Dongfeng. 2024. Cortical lipid metabolic pathway alteration of early Alzheimer's disease and candidate drugs screen. In European journal of medical research, 29, 199. doi:10.1186/s40001-024-01730-w. https://pubmed.ncbi.nlm.nih.gov/38528586/
3. Pei, Zhengtong, Jia, Zhenzhen, Watkins, Paul A. 2005. The second member of the human and murine bubblegum family is a testis- and brainstem-specific acyl-CoA synthetase. In The Journal of biological chemistry, 281, 6632-41. doi:. https://pubmed.ncbi.nlm.nih.gov/16371355/
4. Lopes-Marques, Mónica, Machado, André M, Ruivo, Raquel, Carvalho, Estela, Castro, L Filipe C. 2018. Expansion, retention and loss in the Acyl-CoA synthetase "Bubblegum" (Acsbg) gene family in vertebrate history. In Gene, 664, 111-118. doi:10.1016/j.gene.2018.04.058. https://pubmed.ncbi.nlm.nih.gov/29694909/
5. Xiong, Jinke, Bao, Jingjing, Hu, Wenping, Shang, Mingyu, Zhang, Li. 2023. Whole-genome resequencing reveals genetic diversity and selection characteristics of dairy goat. In Frontiers in genetics, 13, 1044017. doi:10.3389/fgene.2022.1044017. https://pubmed.ncbi.nlm.nih.gov/36685859/
6. Claire D'Andre, Hirwa, Paul, Wallace, Shen, Xu, Sun, Liang, Zhang, Xiquan. 2013. Identification and characterization of genes that control fat deposition in chickens. In Journal of animal science and biotechnology, 4, 43. doi:10.1186/2049-1891-4-43. https://pubmed.ncbi.nlm.nih.gov/24206759/
7. Jiang, Jianping, Liu, Lin, Gao, Yahui, Liang, Weijun, Sun, Dongxiao. 2019. Determination of genetic associations between indels in 11 candidate genes and milk composition traits in Chinese Holstein population. In BMC genetics, 20, 48. doi:10.1186/s12863-019-0751-y. https://pubmed.ncbi.nlm.nih.gov/31138106/
8. Xing, Siyuan, Liu, Ranran, Zhao, Guiping, Crooijmans, Richard P M A, Wen, Jie. 2021. Time Course Transcriptomic Study Reveals the Gene Regulation During Liver Development and the Correlation With Abdominal Fat Weight in Chicken. In Frontiers in genetics, 12, 723519. doi:10.3389/fgene.2021.723519. https://pubmed.ncbi.nlm.nih.gov/34567076/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen