C57BL/6JCya-Slc2a12em1flox/Cya
Common Name:
Slc2a12-flox
Product ID:
S-CKO-10805
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc2a12-flox
Strain ID
CKOCMP-353169-Slc2a12-B6J-VA
Gene Name
Product ID
S-CKO-10805
Gene Alias
GLUT-12; Glut12
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc2a12em1flox/Cya mice (Catalog S-CKO-10805) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042261
NCBI RefSeq
NM_178934
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Slc2a12, also known as GLUT12, is a member of the SLC2 gene family and functions as a glucose transporter. It is involved in pathways related to glucose metabolism, iron homeostasis, and tissue repair [1]. In vertebrates, it has shown to potentially provide functional compensation for the loss of SLC2A4 in birds, and its functionality is associated with basal metabolic rates in endotherms [3].
In hyperuricemia model mice, knockout of Glut12 (encoded by SLC2A12) using the CRISPR-Cas9 system revealed that GLUT12 acts as a physiological urate transporter, and its dysfunction elevates blood urate concentration [4]. In ovarian cancer cells, hypoxia-induced upregulation of SLC2A12 promotes cancer cell growth and metastasis by suppressing ferroptosis. Silencing SLC2A12 in animal models significantly inhibits tumor growth and metastasis [2].
In conclusion, Slc2a12 plays essential roles in glucose and urate transport, as well as in the regulation of cancer cell behavior. Gene-knockout models in mice and in vivo studies in animal models have been crucial in revealing its function in hyperuricemia and ovarian cancer, providing insights into potential disease mechanisms and therapeutic targets.
References：
1. Nauffal, Victor, Di Achille, Paolo, Klarqvist, Marcus D R, Ellinor, Patrick T, Lubitz, Steven A. 2023. Genetics of myocardial interstitial fibrosis in the human heart and association with disease. In Nature genetics, 55, 777-786. doi:10.1038/s41588-023-01371-5. https://pubmed.ncbi.nlm.nih.gov/37081215/
2. Li, Mingmei, Li, Li, Cheng, Xiaoxiao, Li, Longyu, Tu, Kaijia. 2023. Hypoxia promotes the growth and metastasis of ovarian cancer cells by suppressing ferroptosis via upregulating SLC2A12. In Experimental cell research, 433, 113851. doi:10.1016/j.yexcr.2023.113851. https://pubmed.ncbi.nlm.nih.gov/37940066/
3. Xiong, Ying, Lei, Fumin. . SLC2A12 of SLC2 Gene Family in Bird Provides Functional Compensation for the Loss of SLC2A4 Gene in Other Vertebrates. In Molecular biology and evolution, 38, 1276-1291. doi:10.1093/molbev/msaa286. https://pubmed.ncbi.nlm.nih.gov/33316072/
4. Toyoda, Yu, Takada, Tappei, Miyata, Hiroshi, Aiba, Atsu, Suzuki, Hiroshi. 2020. Identification of GLUT12/SLC2A12 as a urate transporter that regulates the blood urate level in hyperuricemia model mice. In Proceedings of the National Academy of Sciences of the United States of America, 117, 18175-18177. doi:10.1073/pnas.2006958117. https://pubmed.ncbi.nlm.nih.gov/32690690/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen