C57BL/6JCya-Edc3em1flox/Cya
Common Name:
Edc3-flox
Product ID:
S-CKO-10811
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Edc3-flox
Strain ID
CKOCMP-353190-Edc3-B6J-VA
Gene Name
Product ID
S-CKO-10811
Gene Alias
Lsm16; Yjdc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Edc3em1flox/Cya mice (Catalog S-CKO-10811) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043990
NCBI RefSeq
NM_153799
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Edc3, or enhancer of mRNA decapping 3, is a crucial protein for P-body assembly. It functions in promoting mRNA decapping and P-body formation, playing a vital role in regulating mRNA stability and translation, which are key processes in determining protein abundance within cells [1,2,3,4,5,6,7,8]. It is involved in various biological pathways related to post-transcriptional control, and its study in genetic models can provide insights into these regulatory mechanisms.
In prostate cancer cells, EDC3 phosphorylation at serine 161 (S161) by Pim1 and 3 protein kinases modifies P-body assembly. A mutation at S161 (EDC3 S161A) leads to decreased cell growth, migration, and invasion, along with reduced expression of integrin β1 and α6 [1]. In PRRSV-infected cells, overexpression of EDC3 inhibits PRRSV proliferation by upregulating MyD88, while knockdown promotes virus transcription and protein expression [3]. In yeast, Edc3 and Scd6 act redundantly with Dhh1 in post-transcriptional repression of starvation-induced pathways, and Edc3 is involved in the decay of specific mRNAs like RPS28B [4,5]. In Candida albicans, the edc3/edc3 deletion strain shows increased stress resistance, lower ROS levels, and decreased CaMCA1 expression [9].
In conclusion, Edc3 is essential for regulating mRNA-related processes such as decapping, P-body formation, and translation. Its dysregulation is associated with cancer-related functions like cell growth and invasion in prostate cancer, and it also impacts viral proliferation in PRRSV-infected cells, stress response in Candida albicans, and starvation-induced pathways in yeast. Studies using loss-of-function models, although not always in KO/CKO mouse models, have significantly enhanced our understanding of these functions and their implications in different biological contexts and disease conditions.
References:
1. Bearss, Jeremiah J, Padi, Sathish Kr, Singh, Neha, Kraft, Andrew S, Okumura, Koichi. 2021. EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics. In EMBO reports, 22, e50835. doi:10.15252/embr.202050835. https://pubmed.ncbi.nlm.nih.gov/33586867/
2. Walters, Robert W, Shumilin, Igor A, Yoon, Je-Hyun, Minor, Wladek, Parker, Roy. 2014. Edc3 function in yeast and mammals is modulated by interaction with NAD-related compounds. In G3 (Bethesda, Md.), 4, 613-22. doi:10.1534/g3.114.010470. https://pubmed.ncbi.nlm.nih.gov/24504254/
3. Wang, Yiran, Li, Changhong, Deng, Qiaomu, Yang, Hongxing, Wen, Guilan. 2025. EDC3 protein of P-body suppresses PRRSV proliferation and functions by upregulating MyD88. In Veterinary microbiology, 302, 110414. doi:10.1016/j.vetmic.2025.110414. https://pubmed.ncbi.nlm.nih.gov/39938412/
4. Kumar, Rakesh, Zhang, Fan, Niphadkar, Shreyas, Laxman, Sunil, Hinnebusch, Alan G. 2024. Decapping activators Edc3 and Scd6 act redundantly with Dhh1 in post-transcriptional repression of starvation-induced pathways. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.28.610059. https://pubmed.ncbi.nlm.nih.gov/39257769/
5. He, Feng, Li, Chunfang, Roy, Bijoyita, Jacobson, Allan. 2014. Yeast Edc3 targets RPS28B mRNA for decapping by binding to a 3' untranslated region decay-inducing regulatory element. In Molecular and cellular biology, 34, 1438-51. doi:10.1128/MCB.01584-13. https://pubmed.ncbi.nlm.nih.gov/24492965/
6. Roy, Raju, Rajyaguru, Purusharth I. 2022. Assay to Study the Phase-transition Behavior of Edc3, a Conserved Processing Body (P-body) Marker Protein. In Bio-protocol, 12, . doi:10.21769/BioProtoc.4487. https://pubmed.ncbi.nlm.nih.gov/36199703/
7. Fromm, Simon A, Truffault, Vincent, Kamenz, Julia, Izaurralde, Elisa, Sprangers, Remco. 2011. The structural basis of Edc3- and Scd6-mediated activation of the Dcp1:Dcp2 mRNA decapping complex. In The EMBO journal, 31, 279-90. doi:10.1038/emboj.2011.408. https://pubmed.ncbi.nlm.nih.gov/22085934/
8. Harigaya, Yuriko, Jones, Brittnee N, Muhlrad, Denise, Gross, John D, Parker, Roy. 2010. Identification and analysis of the interaction between Edc3 and Dcp2 in Saccharomyces cerevisiae. In Molecular and cellular biology, 30, 1446-56. doi:10.1128/MCB.01305-09. https://pubmed.ncbi.nlm.nih.gov/20086104/
9. Jung, Jong-Hwan, Kim, Jinmi. 2014. Roles of Edc3 in the oxidative stress response and CaMCA1-encoded metacaspase expression in Candida albicans. In The FEBS journal, 281, 4841-51. doi:10.1111/febs.13022. https://pubmed.ncbi.nlm.nih.gov/25158786/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen