C57BL/6JCya-Lin28bem1flox/Cya
Common Name:
Lin28b-flox
Product ID:
S-CKO-10848
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Lin28b-flox
Strain ID
CKOCMP-380669-Lin28b-B6J-VA
Gene Name
Product ID
S-CKO-10848
Gene Alias
2810403D23Rik; D030047M17Rik; Lin-28.2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lin28bem1flox/Cya mice (Catalog S-CKO-10848) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000079390
NCBI RefSeq
NM_001031772
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Lin28b, an RNA-binding protein, targets a wide range of microRNAs, modulating their maturation and activity. It is crucial in embryogenic stem cells under normal conditions, blocking differentiation and promoting proliferation. Lin28b can also influence the epithelial-to-mesenchymal transition by repressing let-7 microRNA biogenesis [5].
In breast cancer, Lin28b-high cells promote lung metastasis by building an immune-suppressive pre-metastatic niche. They enable neutrophil recruitment and N2 conversion, and breast cancer-released exosomes with low let-7s, mainly from Lin28b-induced breast cancer stem cells, are essential for this immune suppression [1]. In pancreas cancer, the Lin28b/Wnt5a axis drives the disease through crosstalk between cancer-associated fibroblasts and tumor epithelium [2]. In neuroendocrine prostate cancer, LIN28B transcription is up-regulated, and its depletion by CRISPR inhibits tumorigenesis and xenograft growth, with its functions mediated mainly through let-7 miRNA suppression [3]. In endometrial cancer, LIN28B binds and stabilizes PCAT5, which in turn stabilizes IGF2BP3, promoting aerobic glycolysis, proliferation, migration, and invasion of EC cells [4].
In conclusion, Lin28b plays essential roles in tumor progression and metastasis in multiple solid tumor entities. Model-based research, especially through gene knockout or conditional knockout mouse models, has revealed its significant contributions to breast, pancreas, prostate, and endometrial cancers, highlighting its potential as a therapeutic target and biomarker in these disease areas.
References:
1. Qi, Meiyan, Xia, Yun, Wu, Yanjun, Ji, Weiwei, Zhan, Lixing. 2022. Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression. In Nature communications, 13, 897. doi:10.1038/s41467-022-28438-x. https://pubmed.ncbi.nlm.nih.gov/35173168/
2. Shu, Zhaoqi, Fan, Minghe, Tu, Bo, Shu, Shaokun, Zhao, Ying. 2023. The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium. In Nature communications, 14, 6885. doi:10.1038/s41467-023-42508-8. https://pubmed.ncbi.nlm.nih.gov/37898598/
3. Lovnicki, Jessica, Gan, Yu, Feng, Tingting, Gleave, Martin E, Dong, Xuesen. . LIN28B promotes the development of neuroendocrine prostate cancer. In The Journal of clinical investigation, 130, 5338-5348. doi:10.1172/JCI135373. https://pubmed.ncbi.nlm.nih.gov/32634132/
4. Wang, Bin, Wang, Bo, Ma, Jian, Li, Qing, Ma, Xiao-Xin. 2024. LIN28B induced PCAT5 promotes endometrial cancer progression and glycolysis via IGF2BP3 deubiquitination. In Cell death & disease, 15, 242. doi:10.1038/s41419-024-06564-2. https://pubmed.ncbi.nlm.nih.gov/38565547/
5. Gewalt, Tabea, Noh, Ka-Won, Meder, Lydia. 2023. The role of LIN28B in tumor progression and metastasis in solid tumor entities. In Oncology research, 31, 101-115. doi:10.32604/or.2023.028105. https://pubmed.ncbi.nlm.nih.gov/37304235/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen