C57BL/6JCya-Dgkhem1flox/Cya
Common Name:
Dgkh-flox
Product ID:
S-CKO-10880
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dgkh-flox
Strain ID
CKOCMP-380921-Dgkh-B6J-VA
Gene Name
Product ID
S-CKO-10880
Gene Alias
5930402B05Rik; D130015C16; DGK
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dgkhem1flox/Cya mice (Catalog S-CKO-10880) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000226342
NCBI RefSeq
NM_001081336
Target Region
Exon 9
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Dgkh, coding for the eta (η) isoform of diacylglycerol kinase, is involved in the phosphoinositol pathway [4]. It may have a role in late developmental stages as its expression increases during mouse brain development [4].
In hepatocellular carcinoma (HCC), DGKH is elevated in tumor tissues. Functional assays in DGKH-manipulated HCC cell lines showed that DGKH augmented aggressive features like cancer stemness, therapy resistance, and metastasis. Mechanistically, it promotes mTOR signaling by producing phosphatidic acid, and in an immunocompetent mouse model, cotreatment with sorafenib and liver-directed AAV8-mediated Dgkh depletion reduced tumor burden and related features [1].
In bipolar disorder (BD), DGKH is a replicated risk gene. In a study, the risk haplotype of DGKH was associated with increased amygdala volume in BD patients. Also, the A allele of DGKH (rs9525580) in Japanese healthy volunteers was associated with higher scores of openness to experience, a premorbid personality trait of BD [3,5].
In addition, high glucose in hippocampal neurons can inhibit the demethylation modification of Dgkh by ALKBH5, leading to down-regulation of Dgkh, tau hyperphosphorylation, and potentially diabetic cognitive dysfunction. Overexpression of Dgkh reversed tau hyperphosphorylation in in-vitro and in-vivo models [2].
In conclusion, Dgkh is involved in multiple biological processes and disease conditions. Its role in promoting aggressive features in HCC via mTOR signaling, its association with BD in terms of amygdala volume and personality traits, and its connection to tau hyperphosphorylation in diabetic cognitive dysfunction highlight its importance. Studies on Dgkh, especially those using functional manipulation models, contribute to understanding the mechanisms of these diseases and may offer potential therapeutic targets.
References:
1. Loh, Jia Jian, Ng, Kai Yu, Huang, Ianto Bosheng, Yun, Jing-Ping, Ma, Stephanie. 2024. DGKH-mediated phosphatidic acid oncometabolism as a driver of self-renewal and therapy resistance in HCC. In Hepatology (Baltimore, Md.), , . doi:10.1097/HEP.0000000000001158. https://pubmed.ncbi.nlm.nih.gov/39663958/
2. Qu, Minli, Zuo, Linhui, Zhang, Mengru, Li, Changjun, Wu, Jing. 2023. High glucose induces tau hyperphosphorylation in hippocampal neurons via inhibition of ALKBH5-mediated Dgkh m6A demethylation: a potential mechanism for diabetic cognitive dysfunction. In Cell death & disease, 14, 385. doi:10.1038/s41419-023-05909-7. https://pubmed.ncbi.nlm.nih.gov/37385994/
3. Kittel-Schneider, S, Wobrock, T, Scherk, H, Gruber, O, Reif, A. 2014. Influence of DGKH variants on amygdala volume in patients with bipolar affective disorder and schizophrenia. In European archives of psychiatry and clinical neuroscience, 265, 127-36. doi:10.1007/s00406-014-0513-9. https://pubmed.ncbi.nlm.nih.gov/24958494/
4. Weißflog, Lena, Becker, Nils, Bossert, Nelli, Kittel-Schneider, Sarah, Reif, Andreas. 2016. Expressional profile of the diacylglycerol kinase eta gene DGKH. In European archives of psychiatry and clinical neuroscience, 267, 445-454. doi:10.1007/s00406-016-0695-4. https://pubmed.ncbi.nlm.nih.gov/27085324/
5. Matsumoto, Yoshihiko, Suzuki, Akihito, Shirata, Toshinori, Goto, Kaoru, Otani, Koichi. 2018. Implication of the DGKH genotype in openness to experience, a premorbid personality trait of bipolar disorder. In Journal of affective disorders, 238, 539-541. doi:10.1016/j.jad.2018.06.031. https://pubmed.ncbi.nlm.nih.gov/29936393/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen