C57BL/6JCya-Gpr176em1flox/Cya
Common Name
Gpr176-flox
Product ID
S-CKO-10940
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-381413-Gpr176-B6J-VA
When using this mouse strain in a publication, please cite “Gpr176-flox Mouse (Catalog S-CKO-10940) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gpr176-flox
Strain ID
CKOCMP-381413-Gpr176-B6J-VA
Gene Name
Product ID
S-CKO-10940
Gene Alias
Gm1012
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000039160
NCBI RefSeq
NM_201367
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Overview of Gene Research
Gpr176, a member of the G-protein-coupled receptor superfamily, responds to external stimuli. It can couple to the G-protein subclass Gz (or Gx), participate in reducing cAMP production, and is involved in pathways like cAMP/PKA signaling [1,4]. Gpr176 has significant biological importance in processes such as circadian rhythm regulation in the suprachiasmatic nucleus (SCN) and is also associated with various diseases [3,4]. Genetic mouse models have been crucial for studying its functions.
In cancer, Gpr176-deficiency in genetic mouse models of colorectal cancer demonstrated its role in promoting cancer progression. Gpr176 activates the cAMP/PKA signaling pathway, modulates mitophagy via the GNAS-cAMP/PKA/BNIP3L axis, and promotes tumorigenesis and development of CRC [1]. In organ fibrosis, Gpr176-knockdown in mouse models showed its profibrotic role. In liver fibrosis models (carbon tetrachloride and common bile duct ligation), Gpr176-knockout mice developed less severe fibrosis, suggesting that Gpr176 in hepatic stellate cells is involved in fibrogenesis [2].
In conclusion, Gpr176 plays essential roles in regulating circadian rhythms and has significant impacts on cancer progression and organ fibrosis. Gene-knockout mouse models have been instrumental in uncovering these functions, providing insights into its roles in these disease areas.
References：
1. Tang, Junwei, Peng, Wen, Ji, Jiangzhou, Wang, Xiaowei, Sun, Yueming. 2023. GPR176 Promotes Cancer Progression by Interacting with G Protein GNAS to Restrain Cell Mitophagy in Colorectal Cancer. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2205627. doi:10.1002/advs.202205627. https://pubmed.ncbi.nlm.nih.gov/36905238/
2. De Smet, Vincent, Gürbüz, Elif, Eysackers, Nathalie, Mannaerts, Inge, van Grunsven, Leo A. 2024. Orphan receptor GPR176 in hepatic stellate cells exerts a profibrotic role in chronic liver disease. In JHEP reports : innovation in hepatology, 6, 101036. doi:10.1016/j.jhepr.2024.101036. https://pubmed.ncbi.nlm.nih.gov/38694958/
3. Yamaguchi, Yoshiaki, Murai, Iori, Takeda, Momoko, Miyake, Takahito, Doi, Masao. . Nmu/Nms/Gpr176 Triple-Deficient Mice Show Enhanced Light-Resetting of Circadian Locomotor Activity. In Biological & pharmaceutical bulletin, 45, 1172-1179. doi:10.1248/bpb.b22-00260. https://pubmed.ncbi.nlm.nih.gov/35908898/
4. Nakagawa, Shumpei, Nguyen Pham, Khanh Tien, Shao, Xinyan, Doi, Masao. 2020. Time-Restricted G-Protein Signaling Pathways via GPR176, Gz, and RGS16 Set the Pace of the Master Circadian Clock in the Suprachiasmatic Nucleus. In International journal of molecular sciences, 21, . doi:10.3390/ijms21145055. https://pubmed.ncbi.nlm.nih.gov/32709014/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.