C57BL/6JCya-Rbm33em1flox/Cya
Common Name:
Rbm33-flox
Product ID:
S-CKO-10964
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rbm33-flox
Strain ID
CKOCMP-381626-Rbm33-B6J-VA
Gene Name
Product ID
S-CKO-10964
Gene Alias
3200001K10Rik; 6430512A10Rik; Prr8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbm33em1flox/Cya mice (Catalog S-CKO-10964) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059644
NCBI RefSeq
NM_028234
Target Region
Exon 5
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Rbm33, an RNA-binding protein, has multiple crucial functions. It is involved in regulating the RNA substrate selectivity of m6A demethylase ALKBH5, and also directs the nuclear export of transcripts containing GC-rich elements [1,2]. In terms of associated pathways, it is linked to autophagy regulation and tumorigenesis, especially in head-neck squamous cell carcinoma (HNSCC), and it may be involved in pathways related to extracellular matrix degradation in abdominal aortic aneurysm (AAA) [1,3].
In a KO mouse model study, hippocampus-specific genetic deletion of RBM33 alleviated age-related declines in learning and memory in aged RBM33 knockout (RBM33-/-) mice, while hippocampus-specific overexpression of RBM33 exacerbated these declines in aged RBM33 overexpression (RBM33Tg) mice. This indicates that RBM33 acts as an age-promoting factor in the hippocampus, likely through its liquid-liquid phase separation (LLPS) which is required for its binding to the CDKN1A (p21cip1) promoter, inducing microglial senescence [4].
In conclusion, Rbm33 plays important roles in processes like m6A-mediated mRNA regulation, nuclear export of specific transcripts, tumorigenesis, and hippocampal aging. The Rbm33 KO/overexpression mouse models have revealed its function in hippocampal-dependent cognitive decline, providing insights into potential therapeutic strategies for age-related neurodegenerative diseases.
References：
1. Yu, Fang, Zhu, Allen C, Liu, Shun, He, Chuan, Qian, Zhijian. 2023. RBM33 is a unique m6A RNA-binding protein that regulates ALKBH5 demethylase activity and substrate selectivity. In Molecular cell, 83, 2003-2019.e6. doi:10.1016/j.molcel.2023.05.010. https://pubmed.ncbi.nlm.nih.gov/37257451/
2. Thomas, Anu, Rehfeld, Frederick, Zhang, He, Gillet, Frank, Mendell, Joshua T. 2022. RBM33 directs the nuclear export of transcripts containing GC-rich elements. In Genes & development, 36, 550-565. doi:10.1101/gad.349456.122. https://pubmed.ncbi.nlm.nih.gov/35589130/
3. Wang, Shizhi, Yuan, Qingwen, Zhao, Wenpeng, Zhou, Weimin. 2021. Circular RNA RBM33 contributes to extracellular matrix degradation via miR-4268/EPHB2 axis in abdominal aortic aneurysm. In PeerJ, 9, e12232. doi:10.7717/peerj.12232. https://pubmed.ncbi.nlm.nih.gov/34820156/
4. Yang, Xiaowen, Wei, Lin, Zhong, Saifeng, Zhang, Yonggang, Yu, Aiqing. 2025. Liquid-liquid phase separation of RBM33 facilitates hippocampus aging by inducing microglial senescence by activating CDKN1A. In International journal of biological macromolecules, 310, 142986. doi:10.1016/j.ijbiomac.2025.142986. https://pubmed.ncbi.nlm.nih.gov/40216137/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen