C57BL/6JCya-Cep135em1flox/Cya
Common Name:
Cep135-flox
Product ID:
S-CKO-10968
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cep135-flox
Strain ID
CKOCMP-381644-Cep135-B6J-VA
Gene Name
Product ID
S-CKO-10968
Gene Alias
Cep4; Gm1044
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cep135em1flox/Cya mice (Catalog S-CKO-10968) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000121979
NCBI RefSeq
NM_199032
Target Region
Exon 3~4
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
CEP135, also known as Bld10p in Chlamydomonas reinhardtii, is a conserved centriolar protein. It is essential for constructing the centriole and plays a central role in determining the number of triplets in the centriole independently of the cartwheel [2]. CEP135 is associated with the centrosome, which is the cell's central microtubule-organizing center, and is involved in various cellular processes like the cell cycle, angiogenesis, and cell migration [3].
In breast cancer cells, dysregulation of CEP135 isoforms (CEP135full and CEP135mini) promotes centriole overduplication and contributes to chromosome segregation errors. An increased CEP135full:mini ratio is observed in breast cancer cell lines with high centrosome amplification (CA). Inducing CEP135full expression increases CA, multipolar spindles, anaphase-lagging chromosomes, and micronuclei, while CEP135mini expression reduces centrosome number [1]. In mice, loss of Cep135 in premeiotic germ cells causes oligoasthenoteratozoospermia and male infertility. It leads to abnormal sperm morphology, germ cell apoptosis, and defects in acrosome, flagellum, and head-to-tail connections during spermatogenesis [4].
In conclusion, CEP135 is crucial for centriole structure and function, and its dysregulation is associated with breast cancer and male infertility. The gene knockout mouse models have been instrumental in uncovering its role in spermatogenesis, while studies in cancer cells have shown its importance in maintaining genomic stability, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Ganapathi Sankaran, Divya, Stemm-Wolf, Alexander J, Pearson, Chad G. 2019. CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells. In Molecular biology of the cell, 30, 1230-1244. doi:10.1091/mbc.E18-10-0674. https://pubmed.ncbi.nlm.nih.gov/30811267/
2. Noga, Akira, Horii, Mao, Goto, Yumi, Ishikawa, Takashi, Hirono, Masafumi. 2022. Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel. In The EMBO journal, 41, e104582. doi:10.15252/embj.2020104582. https://pubmed.ncbi.nlm.nih.gov/36093892/
3. Wang, Kun, Wang, Xin, Zhao, Fayun, Wang, Wenzhi, Zhang, Xuejun. . Centrosomal Protein CEP135 Regulates the Migration and Angiogenesis of Endothelial Cells in a Microtubule-Dependent Manner. In Frontiers in bioscience (Landmark edition), 28, 303. doi:10.31083/j.fbl2811303. https://pubmed.ncbi.nlm.nih.gov/38062802/
4. Liu, Hui, Zhang, Haozheng, Qin, Guanghao, Fu, Xiaolong, Gao, Jiangang. 2025. Loss of Cep135 causes oligoasthenoteratozoospermia and male infertility in mice. In Cellular and molecular life sciences : CMLS, 82, 117. doi:10.1007/s00018-025-05616-w. https://pubmed.ncbi.nlm.nih.gov/40095067/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen