C57BL/6JCya-Ephx4em1flox/Cya
Common Name
Ephx4-flox
Product ID
S-CKO-11071
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-384214-Ephx4-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ephx4-flox Mouse (Catalog S-CKO-11071) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ephx4-flox
Strain ID
CKOCMP-384214-Ephx4-B6J-VA
Gene Name
Product ID
S-CKO-11071
Gene Alias
Abhd7, Ephxrp, Gm1382
Background
C57BL/6JCya
Gene Full Name
epoxide hydrolase 4
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 5 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000049146
NCBI Transcript ID
NM_001001804
Target Region
Exon 3~4
Size of Effective Region
~2.3 kb
Overview of Gene Research
EPHX4, a member of the epoxide hydrolase family, though its exact enzymatic function related to epoxide regulation is yet to be fully elucidated, seems to be involved in multiple biological processes. It may play a role in lipid metabolism as it was found localized on the surface of lipid droplets in sebocytes, and its depletion increased lipid droplet size and sebaceous lipogenesis [3]. It has also been implicated in several disease-related pathways, especially in cancer [1,2,4].
In cancer research, EPHX4 has shown significant associations. In laryngeal squamous cell carcinoma, it is highly expressed, associated with poor prognosis, and participates in NK cell-mediated cytotoxicity, while also promoting cancer cell proliferation, colony formation, and invasion [1]. In colorectal cancer, its expression is increased, and it promotes the malignant phenotype of CRC cells. EPHX4 along with other EPHX family members was found to be correlated with some clinicopathologic features and overall survival, and was also positively associated with immune-related genes like CD274, CTLA4, HAVCR2, and TIGIT [2]. In pancreatic adenocarcinoma, EPHX4 is highly expressed, associated with patient sex, clinical stage, and lymph node metastasis, and high expression predicts poor survival. Functional experiments showed its tumor-promoting effects [4].
In conclusion, EPHX4 is involved in lipid metabolism and has tumor-promoting roles in multiple cancers such as laryngeal, colorectal, and pancreatic cancers. These findings from various studies contribute to understanding its biological functions and its potential as a therapeutic target in cancer treatment.
References：
1. Shen, Nimei, Gao, Gang, Lu, Xinhong, Qian, Maohua, Qin, Yang. 2024. Comprehensive analysis of the immune implication of EPHX4 gene in laryngeal squamous cell carcinoma. In Brazilian journal of otorhinolaryngology, 90, 101411. doi:10.1016/j.bjorl.2024.101411. https://pubmed.ncbi.nlm.nih.gov/38663041/
2. Cao, Lichao, Ba, Ying, Chen, Fang, Zhang, Shenrui, Zhang, Hezi. 2025. The prognostic significance of epoxide hydrolases in colorectal cancer. In Biochemistry and biophysics reports, 41, 101912. doi:10.1016/j.bbrep.2024.101912. https://pubmed.ncbi.nlm.nih.gov/39850362/
3. Dahlhoff, Maik, Fröhlich, Thomas, Arnold, Georg J, Zouboulis, Christos C, Schneider, Marlon R. 2014. Characterization of the sebocyte lipid droplet proteome reveals novel potential regulators of sebaceous lipogenesis. In Experimental cell research, 332, 146-55. doi:10.1016/j.yexcr.2014.12.004. https://pubmed.ncbi.nlm.nih.gov/25523620/
4. Huang, Shaoyang, Gu, Dandan, Xiong, Wei. 2025. Exploring the functional and prognostic roles of EPHX4 in pancreatic cancer: Insights from bioinformatics and experimental validation. In Gene, 959, 149504. doi:10.1016/j.gene.2025.149504. https://pubmed.ncbi.nlm.nih.gov/40258406/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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