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C57BL/6NCya-Mir21aem1flox/Cya
Common Name:
Mir21a-flox
Product ID:
S-CKO-11098
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mir21a-flox
Strain ID
CKOCMP-387140-Mir21a-B6N-VA
Gene Name
Mir21a
Product ID
S-CKO-11098
Gene Alias
Mir21; Mirn21; mmu-mir-21; mmu-mir-21a
Background
C57BL/6NCya
NCBI ID
387140
Modification
Conditional knockout
Chromosome
11
Phenotype
MGI:2676885
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mir21aem1flox/Cya mice (Catalog S-CKO-11098) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083521
NCBI RefSeq
NR_029738
Target Region
Exon 1
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mir21a, a microRNA, is involved in numerous biological processes and disease-related pathways. MicroRNAs regulate gene expression at the post-transcriptional level, and Mir21a has been implicated in pathways such as the NF-κB signaling pathway, which is crucial for inflammation and immune response [1,2,4]. It is also associated with cancer-related processes, making it of great biological importance. Genetic models, especially knockout (KO) mouse models, have been instrumental in understanding its function.

In colorectal cancer, Fusobacterium nucleatum activates Toll-like receptor 4 signaling to NF-κB, up-regulating Mir21a expression. Mir21a promotes cell proliferation and tumor development by reducing levels of the RAS GTPase RASA1. MiR21a-/-mice have fewer and smaller colorectal tumors, later appearance of fecal blood and diarrhea, and longer survival times compared to control mice, indicating its pro-tumorigenic role in this context [1]. In glioblastoma, targeting mmu-miR-21a with a single adeno-associated virus vector using CRISPR leads to lowered mmu-miR-21a levels in the brain, reduced tumor growth, and improved overall survival in glioblastoma-bearing mice, suggesting its importance in glioblastoma progression [3].

In conclusion, Mir21a plays essential roles in biological processes, especially in inflammation-related and cancer-associated pathways. Gene knockout mouse models, like the miR21a-/-mice in colorectal cancer and the targeting of mmu-miR-21a in glioblastoma, have significantly contributed to understanding its role in these disease areas, highlighting its potential as a therapeutic target in cancer treatment.

References:
1. Yang, Yongzhi, Weng, Wenhao, Peng, Junjie, Qin, Huanlong, Ma, Yanlei. 2016. Fusobacterium nucleatum Increases Proliferation of Colorectal Cancer Cells and Tumor Development in Mice by Activating Toll-Like Receptor 4 Signaling to Nuclear Factor-κB, and Up-regulating Expression of MicroRNA-21. In Gastroenterology, 152, 851-866.e24. doi:10.1053/j.gastro.2016.11.018. https://pubmed.ncbi.nlm.nih.gov/27876571/
2. Chen, Fei, Xu, Xiao-Yong, Zhang, Ming, Shao, Hong-Tao, Shi, Yi. 2017. Deep sequencing profiles MicroRNAs related to Aspergillus fumigatus infection in lung tissues of mice. In Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 52, 90-99. doi:10.1016/j.jmii.2016.09.002. https://pubmed.ncbi.nlm.nih.gov/30146414/
3. Nieland, Lisa, Vrijmoet, Anne B, Jetten, Isabelle W, Breakefield, Xandra O, Abels, Erik R. 2024. CRISPR targeting of mmu-miR-21a through a single adeno-associated virus vector prolongs survival of glioblastoma-bearing mice. In Molecular therapy : the journal of the American Society of Gene Therapy, 33, 133-151. doi:10.1016/j.ymthe.2024.11.023. https://pubmed.ncbi.nlm.nih.gov/39563028/
4. Georges, Hanah M, Cassin, Caterina, Tong, Mancy, Abrahams, Vikki M. 2024. TLR8-activating miR-146a-3p is an intermediate signal contributing to fetal membrane inflammation in response to bacterial LPS. In Immunology, 172, 577-587. doi:10.1111/imm.13794. https://pubmed.ncbi.nlm.nih.gov/38631842/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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