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C57BL/6NCya-Mir146em1flox/Cya
Common Name:
Mir146-flox
Product ID:
S-CKO-11103
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mir146-flox
Strain ID
CKOCMP-387164-Mir146-B6N-VA
Gene Name
Mir146
Product ID
S-CKO-11103
Gene Alias
Mirn146; miR-146a; mmu-mir-146
Background
C57BL/6NCya
NCBI ID
387164
Modification
Conditional knockout
Chromosome
11
Phenotype
MGI:2676831
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mir146em1flox/Cya mice (Catalog S-CKO-11103) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083667
NCBI RefSeq
NR_029558
Target Region
Exon 1
Size of Effective Region
~1.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mir146, also known as microRNA 146, is a non-coding RNA that plays a significant role in regulating gene expression at the post-transcriptional level. It is involved in various biological processes, such as immune response, inflammation, and cell differentiation. It has been associated with the NF-κB signaling pathway, which is crucial in inflammation and immune regulation [5,6,7]. In the context of disease, Mir146 has implications in cancer, cardiovascular diseases, neurodegenerative diseases, and spermatogenesis [1,2,3,5,6,8].

In mouse models, Mir146 has been shown to modulate spermatogonial differentiation. In undifferentiated spermatogonia, Mir146 levels are high, and its overexpression reduces the levels of mediator complex subunit 1 (Med1) and the differentiation marker Kit. When undifferentiated spermatogonia are exposed to retinoic acid (RA), Mir146 is downregulated, and Kit is upregulated. Overexpressing Mir146 in RA-treated spermatogonia inhibits Kit upregulation, indicating that Mir146 modulates the effects of RA on spermatogonial differentiation [3]. In microglia, Presenilin 2 (PS2) knockout (KO) mice show constitutively down-regulated Mir146. PS2 KO microglia express higher levels of the Mir146 target protein interleukin-1 receptor-associated kinase-1 and have increased NFκB transcriptional activity, suggesting that PS2 impacts microglial responses through modulation of Mir146a [5].

In conclusion, Mir146 is a key regulator in multiple biological processes. Mouse KO models have revealed its role in spermatogonial differentiation and microglial inflammatory responses. These findings have implications for understanding diseases related to spermatogenesis disorders and neurodegenerative diseases. Additionally, its potential as a biomarker in prostate cancer and its role in modulating inflammation in cardiovascular diseases further highlight its importance in disease research [1,2,4,6].

References:
1. Wu, Guanhua, Wang, Da, Xiong, Fei, Chen, Junsheng, Chen, Yongjun. 2024. The emerging roles of CEACAM6 in human cancer (Review). In International journal of oncology, 64, . doi:10.3892/ijo.2024.5615. https://pubmed.ncbi.nlm.nih.gov/38240103/
2. Ginckels, Pieterjan, Holvoet, Paul. 2022. Oxidative Stress and Inflammation in Cardiovascular Diseases and Cancer: Role of Non-coding RNAs. In The Yale journal of biology and medicine, 95, 129-152. doi:. https://pubmed.ncbi.nlm.nih.gov/35370493/
3. Huszar, Jessica M, Payne, Christopher J. 2013. MicroRNA 146 (Mir146) modulates spermatogonial differentiation by retinoic acid in mice. In Biology of reproduction, 88, 15. doi:10.1095/biolreprod.112.103747. https://pubmed.ncbi.nlm.nih.gov/23221399/
4. Worthington, Myla, Aurelus, Chelsey, Banerjee, Narendra, Sarkar, Fazlul, Banerjee, Hirendra Nath. 2022. A Study to Investigate the Role of Noncoding RNA miR146 Alpha as a Potential Biomarker in Prostate Cancer. In Journal of analytical oncology, 11, 21-23. doi:10.30683/1927-7229.2022.11.03. https://pubmed.ncbi.nlm.nih.gov/36540895/
5. Jayadev, Suman, Case, Amanda, Alajajian, Betty, Möller, Thomas, Garden, Gwenn A. 2013. Presenilin 2 influences miR146 level and activity in microglia. In Journal of neurochemistry, 127, 592-9. doi:10.1111/jnc.12400. https://pubmed.ncbi.nlm.nih.gov/23952003/
6. Meng, Wan-Ting, Zhu, Jing, Wang, Ya-Chao, Guo, Hai-Dong, Ji, Guang. 2024. Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury. In Journal of nanobiotechnology, 22, 382. doi:10.1186/s12951-024-02631-0. https://pubmed.ncbi.nlm.nih.gov/38951872/
7. Schober, Andreas, Maleki, Saffiyeh Saboor, Nazari-Jahantigh, Maliheh. . Regulatory Non-coding RNAs in Atherosclerosis. In Handbook of experimental pharmacology, 270, 463-492. doi:10.1007/164_2020_423. https://pubmed.ncbi.nlm.nih.gov/33454857/
8. Volný, Ondřej, Kašičková, Linda, Coufalová, Dominika, Cimflová, Petra, Novák, Jan. . microRNAs in Cerebrovascular Disease. In Advances in experimental medicine and biology, 888, 155-95. doi:10.1007/978-3-319-22671-2_9. https://pubmed.ncbi.nlm.nih.gov/26663183/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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