C57BL/6JCya-Mir148aem1flox/Cya
Common Name:
Mir148a-flox
Product ID:
S-CKO-11104
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mir148a-flox
Strain ID
CKOCMP-387166-Mir148a-B6J-VA
Gene Name
Product ID
S-CKO-11104
Gene Alias
Mirn148; Mirn148a; mir-148a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir148aem1flox/Cya mice (Catalog S-CKO-11104) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083571
NCBI RefSeq
NR_029719
Target Region
Exon 1
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Mir148a, as a microRNA, plays diverse and crucial roles in multiple biological processes. It is part of the MIR148A family along with MIR148B and MIR152, sharing the same seed sequences. Mir148a has been implicated in regulating cell differentiation, with its family promoting cardiomyocyte differentiation from human embryonic stem cells by inhibiting the DLL1-mediated NOTCH signaling pathway, thus repressing paraxial mesodermal differentiation and enhancing lateral mesoderm and cardiomyocyte differentiation [1].
In cancer research, Mir148a exhibits tumor-suppressive properties. In IDH1-mutant gliomas, its promoter is hypermethylated, leading to silencing, and restoration of Mir148a reduces tumorigenic properties, potentially by targeting DNMT1 [2]. In ovarian cancer, overexpression of Mir148a decreases cell proliferation and invasion, partly through inhibition of its target gene TGFI2 [4]. Additionally, in human glioblastoma cells, knockdown of Mir148a combined with baicalin suppresses cell viability, proliferation, and induces apoptosis and autophagy [3].
In summary, Mir148a is involved in regulating cell differentiation and has tumor-suppressive functions in multiple cancers. Research using genetic models, such as loss-of-function experiments, has significantly enhanced our understanding of its roles in these biological processes and disease conditions, providing potential therapeutic targets for related diseases.
References:
1. Fang, Xing, Miao, Shumei, Yu, You, Hu, Shijun, Lei, Wei. 2019. MIR148A family regulates cardiomyocyte differentiation of human embryonic stem cells by inhibiting the DLL1-mediated NOTCH signaling pathway. In Journal of molecular and cellular cardiology, 134, 1-12. doi:10.1016/j.yjmcc.2019.06.014. https://pubmed.ncbi.nlm.nih.gov/31233755/
2. Li, Sichen, Chowdhury, Reshmi, Liu, Fei, Cloughesy, Timothy F, Lai, Albert. 2014. Tumor-suppressive miR148a is silenced by CpG island hypermethylation in IDH1-mutant gliomas. In Clinical cancer research : an official journal of the American Association for Cancer Research, 20, 5808-22. doi:10.1158/1078-0432.CCR-14-0234. https://pubmed.ncbi.nlm.nih.gov/25224277/
3. Paul-Samojedny, Monika, Liduk, Emilia, Kowalczyk, Małgorzata, Suchanek-Raif, Renata, Kowalski, Jan. . The Combination of Baicalin with Knockdown of mir148a Gene Suppresses Cell Viability and Proliferation and Induces the Apoptosis and Autophagy of Human Glioblastoma Multiforme T98G and U87MG Cells. In Current pharmaceutical biotechnology, 24, 686-697. doi:10.2174/1389201023666220627144100. https://pubmed.ncbi.nlm.nih.gov/35761505/
4. Zhao, Min, Su, Zhiying, Zhang, Shiyang, Xie, Yudi, Li, Xiaodong. . ERRATUM. In Oncology research, 25, 155. doi:10.3727/096504017X14811155525280. https://pubmed.ncbi.nlm.nih.gov/28081744/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen