C57BL/6NCya-Cpsf6em1flox/Cya
Common Name:
Cpsf6-flox
Product ID:
S-CKO-11219
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cpsf6-flox
Strain ID
CKOCMP-432508-Cpsf6-B6N-VA
Gene Name
Product ID
S-CKO-11219
Gene Alias
4733401N12Rik; CFIM; CFIM68; HPBRII-4; HPBRII-7
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cpsf6em1flox/Cya mice (Catalog S-CKO-11219) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069168
NCBI RefSeq
NM_001013391
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Cpsf6, also known as Cleavage and polyadenylation specificity factor subunit 6 or CFIm68, is a 68 kDa component of the mammalian cleavage factor I (CFIm) complex. It modulates mRNA alternative polyadenylation (APA), determining 3' untranslated region (UTR) length, an important gene expression control mechanism. Cpsf6 is also involved in the HIV-1 replication cycle, as it directly interacts with the HIV-1 core during infection [4].
In cancer cells, elevated Cpsf6 can lead to a systematic prolongation of 3' UTRs, yet its expression is typically higher in tumors than in healthy tissues. Cpsf6 can undergo liquid-liquid phase separation (LLPS), and elevated LLPS is associated with the preferential usage of the distal poly(A) sites. In lung adenocarcinoma, Cpsf6 elevation is attributed to BRCA1-disrupted R-loop accumulation in its 5' end, which leads to XBP1 3'UTR shortening, attenuating cisplatin-induced ER stress and elevating chemo-resistance. Inhibition of Cpsf6 expression in lung adenocarcinoma cell lines caused a significant reduction in cell proliferation, colony formation, and induced apoptosis. In hepatocellular carcinoma, Cpsf6 is upregulated and promotes tumorigenic activities. Depletion of Cpsf6 suppresses cell viability, colony formation, and the Warburg effect, and inhibits angiogenesis [1,2,3,6,7].
In the context of HIV-1 infection, preventing the formation of Cpsf6 condensates inhibits the infection of wild-type HIV-1. Also, cytoplasmic Cpsf6 regulates HIV-1 capsid trafficking and infection in a cyclophilin A-dependent manner [5,8].
In conclusion, Cpsf6 plays crucial roles in both cancer-related processes such as cell proliferation, apoptosis, chemo-resistance, and metabolism adaption, and in the HIV-1 replication cycle, including viral capsid trafficking and the formation of biomolecular condensates important for infection. Research on Cpsf6, especially through loss-of-function experiments, provides valuable insights into these disease areas, potentially guiding the development of new therapeutic strategies.
References：
1. Liu, Susu, Wu, Runze, Chen, Liutao, Fu, Yonggui, Xu, Anlong. 2023. CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation. In Cell reports, 42, 113197. doi:10.1016/j.celrep.2023.113197. https://pubmed.ncbi.nlm.nih.gov/37777964/
2. Zhu, Chuandong, Xie, Yufeng, Li, Qiang, Yu, Zhengyuan, Chen, Jing. 2023. CPSF6-mediated XBP1 3'UTR shortening attenuates cisplatin-induced ER stress and elevates chemo-resistance in lung adenocarcinoma. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 68, 100933. doi:10.1016/j.drup.2023.100933. https://pubmed.ncbi.nlm.nih.gov/36821972/
3. Zu, Yukun, Wang, Dao, Ping, Wei, Sun, Wei. 2022. The roles of CPSF6 in proliferation, apoptosis and tumorigenicity of lung adenocarcinoma. In Aging, 14, 9300-9316. doi:10.18632/aging.204407. https://pubmed.ncbi.nlm.nih.gov/36446361/
4. Bialas, Katarzyna, Diaz-Griffero, Felipe. 2024. HIV-1-induced translocation of CPSF6 to biomolecular condensates. In Trends in microbiology, 32, 781-790. doi:10.1016/j.tim.2024.01.001. https://pubmed.ncbi.nlm.nih.gov/38267295/
5. Luchsinger, Charlotte, Lee, KyeongEun, Mardones, Gonzalo A, KewalRamani, Vineet N, Diaz-Griffero, Felipe. 2023. Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection. In Scientific reports, 13, 10974. doi:10.1038/s41598-023-37364-x. https://pubmed.ncbi.nlm.nih.gov/37414787/
6. Tan, Sheng, Zhang, Ming, Shi, Xinglong, Sun, Jielin, Zhao, Xiaodong. 2021. CPSF6 links alternative polyadenylation to metabolism adaption in hepatocellular carcinoma progression. In Journal of experimental & clinical cancer research : CR, 40, 85. doi:10.1186/s13046-021-01884-z. https://pubmed.ncbi.nlm.nih.gov/33648552/
7. Sim, Deok Yong, Lee, Hyo-Jung, Ahn, Chi-Hoon, Kim, Bonglee, Kim, Sung-Hoon. 2024. Negative Regulation of CPSF6 Suppresses the Warburg Effect and Angiogenesis Leading to Tumor Progression Via c-Myc Signaling Network: Potential Therapeutic Target for Liver Cancer Therapy. In International journal of biological sciences, 20, 3442-3460. doi:10.7150/ijbs.93462. https://pubmed.ncbi.nlm.nih.gov/38993554/
8. Zhong, Zhou, Ning, Jiying, Boggs, Emerson A, Watkins, Simon C, Ambrose, Zandrea. 2021. Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner. In mBio, 12, . doi:10.1128/mBio.03142-20. https://pubmed.ncbi.nlm.nih.gov/33758083/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen