C57BL/6JCya-Acsl5em1flox/Cya
Common Name:
Acsl5-flox
Product ID:
S-CKO-11250
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Acsl5-flox
Strain ID
CKOCMP-433256-Acsl5-B6J-VA
Gene Name
Product ID
S-CKO-11250
Gene Alias
1700030F05Rik; ACS2; ACS5; Facl5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acsl5em1flox/Cya mice (Catalog S-CKO-11250) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043150
NCBI RefSeq
NM_027976
Target Region
Exon 4~5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Acsl5, or acyl-CoA synthetase long-chain family member 5, is a nuclear-coded gene. It is localized in the endoplasmic reticulum and mitochondrial outer membrane, where it catalyzes the formation of fatty acyl-CoAs from long-chain fatty acids (C16-C20). These fatty acyl-CoAs are then used in lipid synthesis or β-oxidation mediated pathways, playing a crucial role in fatty acid metabolism [2].
In non-alcoholic fatty liver disease (NAFLD), high-fat diet-induced NAFLD is suppressed by ACSL5 hepatic overexpression but exacerbated by its depletion. Cytoplasmic SIRT6-mediated ACSL5 deacetylation facilitates hepatic fatty acid oxidation, thus impeding NAFLD progression [1]. In acute myeloid leukemia (AML), ACSL5 knockdown in AML cells inhibits cell growth both in vitro and in vivo, and its level can serve as an independent prognostic predictor of overall survival of AML patients. ACSL5 modulates the activity of Wnt/β-catenin signaling by palmitoylation modification [3]. In the small intestine, intestine-specific ACSL5 deficiency (ACSL5IKO) in mice reduces food intake and protects against diet-induced obesity by potentiating postprandial GLP-1 & PYY secretion [4].
In conclusion, Acsl5 is essential for fatty acid metabolism. Model-based research, especially gene knockout (KO) or conditional knockout (CKO) mouse models, has revealed its roles in various disease conditions such as NAFLD, AML, and diet-induced obesity, providing insights into potential therapeutic strategies for these diseases.
References：
1. Hou, Tianyun, Tian, Yuan, Cao, Ziyang, Yang, Yang, Zhu, Wei-Guo. 2022. Cytoplasmic SIRT6-mediated ACSL5 deacetylation impedes nonalcoholic fatty liver disease by facilitating hepatic fatty acid oxidation. In Molecular cell, 82, 4099-4115.e9. doi:10.1016/j.molcel.2022.09.018. https://pubmed.ncbi.nlm.nih.gov/36208627/
2. Luo, Qin, Das, Avash, Oldoni, Federico, Luo, Fei, Fang, Zhenfei. 2023. Role of ACSL5 in fatty acid metabolism. In Heliyon, 9, e13316. doi:10.1016/j.heliyon.2023.e13316. https://pubmed.ncbi.nlm.nih.gov/36816310/
3. Ye, Wenle, Wang, Jinghan, Huang, Jiansong, Sun, Jie, Jin, Jie. 2023. ACSL5, a prognostic factor in acute myeloid leukemia, modulates the activity of Wnt/β-catenin signaling by palmitoylation modification. In Frontiers of medicine, 17, 685-698. doi:10.1007/s11684-022-0942-1. https://pubmed.ncbi.nlm.nih.gov/37131085/
4. Griffin, John D, Zhu, Ying, Reeves, Andrew, Buhman, Kimberly K, Greenberg, Andrew S. 2024. Intestinal Acyl-CoA synthetase 5 (ACSL5) deficiency potentiates postprandial GLP-1 & PYY secretion, reduces food intake, and protects against diet-induced obesity. In Molecular metabolism, 83, 101918. doi:10.1016/j.molmet.2024.101918. https://pubmed.ncbi.nlm.nih.gov/38499083/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen