C57BL/6JCya-Hs6st1em1flox/Cya
Common Name:
Hs6st1-flox
Product ID:
S-CKO-11397
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hs6st1-flox
Strain ID
CKOCMP-50785-Hs6st1-B6J-VA
Gene Name
Product ID
S-CKO-11397
Gene Alias
6Ost1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hs6st1em1flox/Cya mice (Catalog S-CKO-11397) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000088174
NCBI RefSeq
NM_015818
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Hs6st1, also known as heparan sulfate 6-O-sulfotransferase 1, is an enzyme crucial for heparan sulfate (HS) modification. HS is an extracellular component essential for regulating morphogen gradients by modulating morphogen binding. Hs6st1 is involved in multiple biological processes and signaling pathways, such as those related to neuroectodermal patterning, and is associated with diseases like delayed puberty and Kallmann syndrome [1,2,3]. Genetic models, like KO mouse models, are valuable for studying its functions.
In Xenopus, Hs6st1 knockout using the Nuclease technology system expands the neural plate region, followed by retinal malformation, indicating its role in neuroectodermal patterning by selectively regulating morphogen distribution. In mice, Hs6st1 knockout leads to severe corpus callosum phenotypes, with a hyperactivation of Erk signaling in the embryonic telencephalon, and this phenotype can be rescued by suppressing Fgf8/Erk axis components. Also, in mice, heterozygous Hs6st1 loss causes self-limited delayed puberty [1,4,2].
In conclusion, Hs6st1 is vital for regulating morphogen distribution in processes like neuroectodermal patterning and for proper development of the corpus callosum. Its insufficiency is linked to self-limited delayed puberty. The study of Hs6st1 using KO mouse models has provided insights into its role in these biological processes and disease conditions, enhancing our understanding of related mechanisms.
References:
1. Yamamoto, Takayoshi, Kaneshima, Toki, Tsukano, Kohei, Michiue, Tatsuo. 2023. The heparan sulfate modification enzyme, Hs6st1, governs Xenopus neuroectodermal patterning by regulating distributions of Fgf and Noggin. In Developmental biology, 496, 87-94. doi:10.1016/j.ydbio.2023.01.011. https://pubmed.ncbi.nlm.nih.gov/36739958/
2. Howard, Sasha R, Oleari, Roberto, Poliandri, Ariel, Cariboni, Anna, Dunkel, Leo. . HS6ST1 Insufficiency Causes Self-Limited Delayed Puberty in Contrast With Other GnRH Deficiency Genes. In The Journal of clinical endocrinology and metabolism, 103, 3420-3429. doi:10.1210/jc.2018-00646. https://pubmed.ncbi.nlm.nih.gov/29931354/
3. Moon, Sohyun, Zhao, Ying-Tao. . Convergent biological pathways underlying the Kallmann syndrome-linked genes Hs6st1 and Fgfr1. In Human molecular genetics, 31, 4207-4216. doi:10.1093/hmg/ddac172. https://pubmed.ncbi.nlm.nih.gov/35899427/
4. Clegg, James M, Conway, Christopher D, Howe, Kathy M, Basson, M Albert, Pratt, Thomas. . Heparan sulfotransferases Hs6st1 and Hs2st keep Erk in check for mouse corpus callosum development. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 34, 2389-401. doi:10.1523/JNEUROSCI.3157-13.2014. https://pubmed.ncbi.nlm.nih.gov/24501377/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen