C57BL/6JCya-Keap1em1flox/Cya
Common Name:
Keap1-flox
Product ID:
S-CKO-11409
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Keap1-flox
Strain ID
CKOCMP-50868-Keap1-B6J-VA
Gene Name
Product ID
S-CKO-11409
Gene Alias
INRF2; mKIAA0132
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Keap1em1flox/Cya mice (Catalog S-CKO-11409) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000164812
NCBI RefSeq
NM_016679
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Keap1, also known as Kelch-like ECH-associated protein 1, is a crucial component in the Nrf2-Keap1 signaling pathway [1-10]. It acts as an endogenous inhibitor of Nrf2, a transcription factor. Under normal conditions, Keap1 binds to Nrf2, targeting it for proteasomal degradation. However, in the presence of oxidative or electrophilic stress, this interaction is disrupted, allowing Nrf2 to translocate to the nucleus and activate the transcription of cytoprotective genes [1-5, 7-9]. This system is a fundamental intracellular defense mechanism against oxidative stress, conserved evolutionarily, and is involved in maintaining redox homeostasis [1,2].
In breast cancer, Keap1 has been shown to be a positive prognostic factor. It regulates cell proliferation, apoptosis, and cell cycle transition, and controls mitochondrial biogenesis by promoting the degradation of HSPA9 through ubiquitination [3]. In sepsis, a small-molecule inhibitor of the Keap1-Nrf2 interaction, IR-61, preferentially accumulates in macrophages at infection sites. By directly inhibiting the Keap1-Nrf2 interaction, it activates Nrf2, enhancing the antibacterial function of macrophages, bacterial clearance, and improving sepsis outcomes in mouse models [4].
In conclusion, Keap1 plays a vital role in the Nrf2-Keap1 signaling pathway, which is essential for protecting cells from oxidative stress. Studies using models such as mouse models in breast cancer and sepsis have revealed its significance in disease-related biological processes. These findings contribute to understanding the role of Keap1 in specific disease areas, potentially providing new therapeutic targets for treatment [3,4].
References:
1. Bellezza, Ilaria, Giambanco, Ileana, Minelli, Alba, Donato, Rosario. 2018. Nrf2-Keap1 signaling in oxidative and reductive stress. In Biochimica et biophysica acta. Molecular cell research, 1865, 721-733. doi:10.1016/j.bbamcr.2018.02.010. https://pubmed.ncbi.nlm.nih.gov/29499228/
2. Yamamoto, Masayuki, Kensler, Thomas W, Motohashi, Hozumi. . The KEAP1-NRF2 System: a Thiol-Based Sensor-Effector Apparatus for Maintaining Redox Homeostasis. In Physiological reviews, 98, 1169-1203. doi:10.1152/physrev.00023.2017. https://pubmed.ncbi.nlm.nih.gov/29717933/
3. Han, Bing, Zhen, Fang, Sun, Yue, Li, Shui-Jie, Hu, Jing. 2024. Tumor suppressor KEAP1 promotes HSPA9 degradation, controlling mitochondrial biogenesis in breast cancer. In Cell reports, 43, 114507. doi:10.1016/j.celrep.2024.114507. https://pubmed.ncbi.nlm.nih.gov/39003742/
4. Wang, Yawei, Tang, Binlin, Li, Huijuan, Wang, Yu, Shi, Chunmeng. 2023. A small-molecule inhibitor of Keap1-Nrf2 interaction attenuates sepsis by selectively augmenting the antibacterial defence of macrophages at infection sites. In EBioMedicine, 90, 104480. doi:10.1016/j.ebiom.2023.104480. https://pubmed.ncbi.nlm.nih.gov/36863256/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen