C57BL/6JCya-Exosc10em1flox/Cya
Common Name:
Exosc10-flox
Product ID:
S-CKO-11424
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Exosc10-flox
Strain ID
CKOCMP-50912-Exosc10-B6J-VA
Gene Name
Product ID
S-CKO-11424
Gene Alias
PM-Scl; PM/Scl-100; Pmscl2; RRP6; p2; p3; p4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Exosc10em1flox/Cya mice (Catalog S-CKO-11424) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000017408
NCBI RefSeq
NM_016699
Target Region
Exon 4~6
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Exosc10, also known as Rrp6, is a conserved 3'-5' exoribonuclease. It is part of the multimeric nuclear RNA exosome, processing and degrading RNA, regulating gene expression, and participating in DNA double-strand break repair and telomere maintenance [4]. It is involved in various biological processes such as gametogenesis, embryo development, and ribosome biogenesis.
Gene knockout (KO) and conditional knockout (CKO) mouse models have been crucial in revealing Exosc10's functions. Exosc10-/-mice show embryonic lethality, with mutants arresting at the eight-cell embryo/morula transition, indicating its essential role in early embryogenesis [1]. In oocytes, Exosc10-specific inactivation leads to female subfertility due to delayed germinal vesicle breakdown and oocyte development/maturation impairment, ultimately depleting the ovarian reserve [5,6]. In the initial segment of the epididymis, Exosc10 deficiency is dispensable for sperm maturation and male fertility [3]. In hepatocellular carcinoma, high EXOSC10 expression is an independent prognostic factor, and its elimination inhibits cell proliferation and migration while inducing apoptosis, potentially regulating HCC progression through the p53-related signaling pathway [2].
In conclusion, Exosc10 plays essential roles in early embryogenesis, oogenesis, and is associated with hepatocellular carcinoma prognosis. The KO and CKO mouse models have significantly contributed to understanding its functions in these biological processes and disease conditions, providing insights into potential therapeutic targets for related diseases.
References:
1. Petit, Fabrice G, Jamin, Soazik P, Kernanec, Pierre-Yves, Halet, Guillaume, Primig, Michael. 2021. EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition. In Developmental biology, 483, 58-65. doi:10.1016/j.ydbio.2021.12.010. https://pubmed.ncbi.nlm.nih.gov/34965385/
2. Meng, Zhi-Yong, Fan, Yu-Chun, Zhang, Chao-Sheng, Chen, Chuang, Jiang, Li-He. 2023. EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification. In PeerJ, 11, e15860. doi:10.7717/peerj.15860. https://pubmed.ncbi.nlm.nih.gov/37701829/
3. Zhou, Meiyang, Yu, Junjie, Xu, Yu, Li, Nana, Wang, Zhengpin. 2024. Exosc10 deficiency in the initial segment is dispensable for sperm maturation and male fertility in mice. In Zygote (Cambridge, England), 32, 437-445. doi:10.1017/S0967199424000418. https://pubmed.ncbi.nlm.nih.gov/39552503/
4. Stuparević, Igor, Novačić, Ana, Rahmouni, A Rachid, Lamb, Ned, Primig, Michael. 2021. Regulation of the conserved 3'-5' exoribonuclease EXOSC10/Rrp6 during cell division, development and cancer. In Biological reviews of the Cambridge Philosophical Society, 96, 1092-1113. doi:10.1111/brv.12693. https://pubmed.ncbi.nlm.nih.gov/33599082/
5. Wu, Di, Dean, Jurrien. . EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes. In Nucleic acids research, 48, 5349-5365. doi:10.1093/nar/gkaa249. https://pubmed.ncbi.nlm.nih.gov/32313933/
6. Demini, Leïla, Kervarrec, Christine, Guillot, Laëtitia, Petit, Fabrice G, Jamin, Soazik P. 2023. Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice. In International journal of biological sciences, 19, 1080-1093. doi:10.7150/ijbs.72889. https://pubmed.ncbi.nlm.nih.gov/36923944/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen