C57BL/6JCya-Pbkem1flox/Cya
Common Name:
Pbk-flox
Product ID:
S-CKO-11475
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Pbk-flox
Strain ID
CKOCMP-52033-Pbk-B6J-VA
Gene Name
Product ID
S-CKO-11475
Gene Alias
2810434B10Rik; D14Ertd732e; TOPK
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pbkem1flox/Cya mice (Catalog S-CKO-11475) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022612
NCBI RefSeq
NM_023209
Target Region
Exon 3~5
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
PBK, also known as PDZ-binding kinase or TOPK (T-lymphokine-activated killer-cell-originated protein kinase), is a serine/threonine protein kinase associated with the dual-specific mitogen-activated protein kinase (MAPKK) family. It plays crucial roles in mitosis and cell-cycle progression, especially in proliferative cells. PBK is involved in multiple signaling pathways such as MAPK, PI3K/PTEN/AKT, and NOTCH1, and its abnormal overexpression is associated with the development, progression, and metastasis of malignancies [2,3].
In ovarian cancer, overexpression of PBK contributes to olaparib resistance. Knockdown of PBK in olaparib-resistant SKOV3 cells sensitizes them to olaparib, and inhibition of PBK with a specific inhibitor enhances olaparib's therapeutic efficiency. Mechanistically, PBK directly interacts with TRIM37 to promote its phosphorylation and nuclear translocation, activating the NFκB pathway [1]. In cervical cancer, PBK is highly expressed and promotes cell proliferation, metastasis, and cisplatin resistance. The PBK inhibitor OTS514 can suppress these malignant phenotypes in vitro and in a xenograft model by targeting the ERK/c-Myc signaling pathway [4].
In conclusion, PBK is a key regulator in cell-cycle-related biological processes and is significantly associated with cancer-related diseases. Studies using gene-targeting methods in cell lines and in vivo models, such as those with PBK inhibitors or knockdown, have revealed its role in conferring drug resistance and promoting cancer progression, suggesting that PBK could be a potential therapeutic target for cancer treatment.
References:
1. Ma, Hanlin, Qi, Gonghua, Han, Fang, Yuan, Cunzhong, Kong, Beihua. 2022. PBK drives PARP inhibitor resistance through the TRIM37/NFκB axis in ovarian cancer. In Experimental & molecular medicine, 54, 999-1010. doi:10.1038/s12276-022-00809-w. https://pubmed.ncbi.nlm.nih.gov/35859118/
2. Han, Ziping, Li, Lingzhi, Huang, Yuyou, Zhao, Haiping, Luo, Yumin. 2021. PBK/TOPK: A Therapeutic Target Worthy of Attention. In Cells, 10, . doi:10.3390/cells10020371. https://pubmed.ncbi.nlm.nih.gov/33670114/
3. Huang, Hai, Lee, Mee-Hyun, Liu, Kangdong, Ryoo, Zeayoung, Kim, Myoung Ok. 2021. PBK/TOPK: An Effective Drug Target with Diverse Therapeutic Potential. In Cancers, 13, . doi:10.3390/cancers13092232. https://pubmed.ncbi.nlm.nih.gov/34066486/
4. Ma, Hanlin, Han, Fang, Yan, Xiaohui, Song, Kun, Kong, Beihua. 2020. PBK promotes aggressive phenotypes of cervical cancer through ERK/c-Myc signaling pathway. In Journal of cellular physiology, 236, 2767-2781. doi:10.1002/jcp.30134. https://pubmed.ncbi.nlm.nih.gov/33184870/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen