C57BL/6JCya-Parp8em1flox/Cya
Common Name:
Parp8-flox
Product ID:
S-CKO-11524
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Parp8-flox
Strain ID
CKOCMP-52552-Parp8-B6J-VA
Gene Name
Product ID
S-CKO-11524
Gene Alias
2810430O08Rik; ARTD16; D13Ertd275e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Parp8em1flox/Cya mice (Catalog S-CKO-11524) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022239
NCBI RefSeq
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Target Region
Exon 5~6
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
PARP8, also known as poly (ADP-ribose) polymerase family member 8, is involved in multiple biological processes. Although the references don't elaborate on its core function, it may be related to DNA repair as suggested by its association with altitude adaptation in Tibetan and Dahe pigs where genes related to DNA repair were identified [2]. It may also play a role in immunogenic cell death as its down-regulation led to decreased cell proliferation and slower migration of uveal melanoma cells [6].
Genetic studies have identified PARP8 in various contexts. In human immunity, high-scoring signals of positive selection were detected at PARP8, and interactions with HIV-1 and SARS-CoV-2 were identified, suggesting its role in the immune response [1]. In prostate cancer, the deletion of PARP8 was correlated with progression-free survival independent of disease stage and Gleason prognostic group grade, indicating its potential as a prognostic marker [3]. In high myopia, a missense variant in PARP8 was detected, suggesting it as a new candidate disease-causing gene [4]. In leukemia, PARP8 was prioritized as a candidate target downstream of MLL-AF9 [5]. In severe acne, differential methylation and expression of PARP8 were found between severe acne and health control groups [7]. In differentiating latent from active tuberculosis in children, PARP8 was identified as one of the hub genes related to ferroptosis, potentially serving as a biomarker [8]. In ulcerative colitis, PARP8 was identified as one of the hub genes related to ferroptosis, capable of distinguishing UC patients from controls [9]. In uveal melanoma, PARP8 was part of a glycosylation-based gene signature for prognostic prediction [10].
In summary, PARP8 appears to be involved in a diverse range of biological processes and disease conditions, including immunity, cancer, myopia, and inflammatory diseases. Its identification in these various contexts through genetic analysis provides valuable insights into its potential functions and implications in human health and disease.
References:
1. Urnikyte, Alina, Masiulyte, Abigaile, Pranckeniene, Laura, Kučinskas, Vaidutis. 2023. Disentangling archaic introgression and genomic signatures of selection at human immunity genes. In Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 116, 105528. doi:10.1016/j.meegid.2023.105528. https://pubmed.ncbi.nlm.nih.gov/37977419/
2. Dong, Kunzhe, Yao, Na, Pu, Yabin, Rao, Shaoqi, Ma, Yuehui. 2014. Genomic scan reveals loci under altitude adaptation in Tibetan and Dahe pigs. In PloS one, 9, e110520. doi:10.1371/journal.pone.0110520. https://pubmed.ncbi.nlm.nih.gov/25329542/
3. Alfahed, Abdulaziz, Ebili, Henry Okuchukwu, Almoammar, Nasser Eissa, Abdulwahed, Abdulhadi M, Waggiallah, Hisham Ali. 2023. Prognostic Values of Gene Copy Number Alterations in Prostate Cancer. In Genes, 14, . doi:10.3390/genes14050956. https://pubmed.ncbi.nlm.nih.gov/37239316/
4. Liu, Yang, Zhang, Jin-Jin, Piao, Shun-Yu, Jin, Zi-Bing, Zhuang, Wen-Juan. 2021. Whole-Exome Sequencing in a Cohort of High Myopia Patients in Northwest China. In Frontiers in cell and developmental biology, 9, 645501. doi:10.3389/fcell.2021.645501. https://pubmed.ncbi.nlm.nih.gov/34222226/
5. Fleischmann, Katrin K, Pagel, Philipp, Schmid, Irene, Roscher, Adelbert A. 2014. RNAi-mediated silencing of MLL-AF9 reveals leukemia-associated downstream targets and processes. In Molecular cancer, 13, 27. doi:10.1186/1476-4598-13-27. https://pubmed.ncbi.nlm.nih.gov/24517546/
6. Li, Xiaoyan, Kang, Jing, Yue, Jing, Wang, Lin, Li, Guoyin. 2023. Identification and validation of immunogenic cell death-related score in uveal melanoma to improve prediction of prognosis and response to immunotherapy. In Aging, 15, 3442-3464. doi:10.18632/aging.204680. https://pubmed.ncbi.nlm.nih.gov/37142279/
7. Wang, Huai, Dang, Tianyuan, Feng, Jiaqi, He, Li, Yang, Jiankang. . Identification of differentially methylated genes for severe acne by genome-wide DNA methylation and gene expression analysis. In Epigenetics, 18, 2199373. doi:10.1080/15592294.2023.2199373. https://pubmed.ncbi.nlm.nih.gov/37018476/
8. Chen, Liang, Hua, Jie, Dai, Xiaoting, He, Xiaopu. . Assessment of ferroptosis-associated gene signatures as potential biomarkers for differentiating latent from active tuberculosis in children. In Microbial genomics, 9, . doi:10.1099/mgen.0.000997. https://pubmed.ncbi.nlm.nih.gov/37163321/
9. Qian, Rui, Tang, Min, Ouyang, Zichen, Cheng, Honghui, Xing, Sizhong. . Identification of ferroptosis-related genes in ulcerative colitis: a diagnostic model with machine learning. In Annals of translational medicine, 11, 177. doi:10.21037/atm-23-276. https://pubmed.ncbi.nlm.nih.gov/36923072/
10. Liu, Jianlan, Zhang, Pengpeng, Yang, Fang, Yao, Gang, Tang, Jian. 2023. Integrating single-cell analysis and machine learning to create glycosylation-based gene signature for prognostic prediction of uveal melanoma. In Frontiers in endocrinology, 14, 1163046. doi:10.3389/fendo.2023.1163046. https://pubmed.ncbi.nlm.nih.gov/37033251/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen