C57BL/6JCya-Cbx7em1flox/Cya
Common Name:
Cbx7-flox
Product ID:
S-CKO-11532
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cbx7-flox
Strain ID
CKOCMP-52609-Cbx7-B6J-VA
Gene Name
Product ID
S-CKO-11532
Gene Alias
1600014J01Rik; D15Ertd417e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cbx7em1flox/Cya mice (Catalog S-CKO-11532) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109616
NCBI RefSeq
NM_144811
Target Region
Exon 3~4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Cbx7, or Chromobox protein homolog 7, is a member of the Chromobox protein family and participates in the formation of the polycomb repressive complex 1(PRC1). It often acts as an epigenetic regulator to regulate gene expression in cells and is involved in various biological processes, such as the cell cycle, immune response, and DNA repair [1].
In cardiomyocytes, Cbx7 overexpression reduces neonatal cardiomyocyte proliferation and promotes multinucleation, while genetic inactivation of Cbx7 increases cardiomyocyte proliferation and promotes cardiac regeneration in neonatal and adult injured hearts. Mechanistically, Cbx7 interacts with TARDBP and positively regulates its downstream target, RBM38 [2]. In basal-like breast cancer, Cbx7 is down-regulated, and it suppresses cancer growth and metastasis through the Twist1/EphA2 pathway [3]. In urinary bladder cancer, down-regulation of Cbx7 due to promoter hypermethylation is correlated with poor prognosis, and its ectopic expression suppresses cancer cell proliferation, migration, invasion, and cancer stemness [4]. In meningioma, Cbx7 expression decreases with malignancy grade, and its restoration induces cell cycle arrest and inhibits cell proliferation by reprogramming metabolic flux [5]. In ovarian cancer, adipose-derived exosomal miR-421 targets Cbx7 and promotes metastatic potential [6]. In liver regeneration, silencing Cbx7 promotes hepatocyte proliferation, increases the liver/body weight ratio, and enhances the Ki67-positive cell count after hepatectomy [7].
In conclusion, Cbx7 plays crucial roles in various biological processes and disease conditions. Its functions in cell cycle regulation, cancer progression, and tissue regeneration are well-demonstrated through in vivo studies using gene knockout or conditional knockout mouse models. These models have provided valuable insights into the role of Cbx7 in specific disease areas such as cancer, cardiac and liver diseases, highlighting its potential as a therapeutic target.
References:
1. Li, Jun, Ouyang, Taohui, Li, Meihua, Meng, Wei, Zhang, Na. 2021. CBX7 is Dualistic in Cancer Progression Based on its Function and Molecular Interactions. In Frontiers in genetics, 12, 740794. doi:10.3389/fgene.2021.740794. https://pubmed.ncbi.nlm.nih.gov/34659360/
2. Cho, Kyu-Won, Andrade, Mark, Bae, Seongho, Park, Changwon, Yoon, Young-Sup. 2023. Polycomb Group Protein CBX7 Represses Cardiomyocyte Proliferation Through Modulation of the TARDBP/RBM38 Axis. In Circulation, 147, 1823-1842. doi:10.1161/CIRCULATIONAHA.122.061131. https://pubmed.ncbi.nlm.nih.gov/37158107/
3. Dai, Tao, Liu, Yiqi, Cao, Renxian, Cao, Jingying. 2022. CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway. In Translational oncology, 24, 101468. doi:10.1016/j.tranon.2022.101468. https://pubmed.ncbi.nlm.nih.gov/35843065/
4. Huang, Zhengnan, Yan, Yilin, Zhu, Zhen, Shen, Bing, Yan, Jun. 2021. CBX7 suppresses urinary bladder cancer progression via modulating AKR1B10-ERK signaling. In Cell death & disease, 12, 537. doi:10.1038/s41419-021-03819-0. https://pubmed.ncbi.nlm.nih.gov/34035231/
5. Cheng, Haixia, Hua, Lingyang, Tang, Hailiang, Wang, Xianli, Gong, Ye. . CBX7 reprograms metabolic flux to protect against meningioma progression by modulating the USP44/c-MYC/LDHA axis. In Journal of molecular cell biology, 15, . doi:10.1093/jmcb/mjad057. https://pubmed.ncbi.nlm.nih.gov/37791390/
6. Zhang, Yi, Tedja, Roslyn, Millman, Michael, Mor, Gil, Alvero, Ayesha B. 2023. Adipose-derived exosomal miR-421 targets CBX7 and promotes metastatic potential in ovarian cancer cells. In Journal of ovarian research, 16, 233. doi:10.1186/s13048-023-01312-0. https://pubmed.ncbi.nlm.nih.gov/38037081/
7. Dou, Zhimin, Lu, Fei, Hu, Jinjing, Li, Bin, Li, Xun. 2024. CBX7 silencing promoted liver regeneration by interacting with BMI1 and activating the Nrf2/ARE signaling pathway. In Scientific reports, 14, 11008. doi:10.1038/s41598-024-58248-8. https://pubmed.ncbi.nlm.nih.gov/38744845/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen