C57BL/6NCya-E2f7em1flox/Cya
Common Name:
E2f7-flox
Product ID:
S-CKO-11546
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
E2f7-flox
Strain ID
CKOCMP-52679-E2f7-B6N-VA
Gene Name
Product ID
S-CKO-11546
Gene Alias
A630014C11Rik; D10Ertd739e; E2F-7
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-E2f7em1flox/Cya mice (Catalog S-CKO-11546) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000073781
NCBI RefSeq
NM_178609
Target Region
Exon 4
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
E2f7 is a recently discovered member of the E2F family. It has essential functions in embryonic development, angiogenesis, and the nervous system [1]. E2f7 is also involved in various signaling pathways, such as the PTEN/AKT/mTOR pathway, and is associated with the development of many diseases, especially cancers [2].
In glioblastoma, E2F7 is up-regulated, promoting cell proliferation, cell-cycle progression, cell metastasis, and tumorigenicity both in vitro and in vivo through the E2F7-EZH2 axis regulating the PTEN/AKT/mTOR signalling [2]. In hepatocellular carcinoma, E2F7 enhances tumor growth by preserving the SP1/SOX4/Anillin axis via repressing miRNA-383-5p transcription and promotes mTOR inhibitor resistance [3,4]. In endometrial cancer, the E2F7/RAD51AP1 axis inhibits cell sensitivity to 5-FU via the fatty acid metabolic pathway [5]. In nasopharyngeal carcinoma, VIRMA promotes tumorigenesis and metastasis by up-regulating E2F7 in an m6A-dependent manner [6]. In lung adenocarcinoma, E2F7 may serve as a potential prognostic biomarker and is up-regulated, potentially affecting tumorigenesis, invasion, and metastasis by regulating DNA repair, damage, and cell cycle processes [7].
In conclusion, E2F7 plays significant roles in multiple biological processes and is closely associated with the occurrence and development of various cancers. Studies on E2F7 in different cancer models have provided insights into its functions in disease, suggesting its potential as a therapeutic target for cancer treatment [1].
References:
1. Chen, Ke-Qian, Lei, Hai-Bo, Liu, Xiang, Wang, Shu-Zhi. 2024. The roles of E2F7 in cancer: Current knowledge and future prospects. In Heliyon, 10, e34362. doi:10.1016/j.heliyon.2024.e34362. https://pubmed.ncbi.nlm.nih.gov/39108857/
2. Yang, Rui, Wang, Mei, Zhang, Guanghui, Yang, Wancai, Cui, Hongjuan. 2020. E2F7-EZH2 axis regulates PTEN/AKT/mTOR signalling and glioblastoma progression. In British journal of cancer, 123, 1445-1455. doi:10.1038/s41416-020-01032-y. https://pubmed.ncbi.nlm.nih.gov/32814835/
3. Hao, Fengjie, Wang, Nan, Zhang, Yifan, Fei, Xiaochun, Wang, Junqing. 2022. E2F7 enhances hepatocellular carcinoma growth by preserving the SP1/SOX4/Anillin axis via repressing miRNA-383-5p transcription. In Molecular carcinogenesis, 61, 975-988. doi:10.1002/mc.23454. https://pubmed.ncbi.nlm.nih.gov/35924788/
4. Ling, Sunbin, Zhan, Qifan, Jiang, Guangjiang, Xiang, Jiajia, Xu, Xiao. 2022. E2F7 promotes mammalian target of rapamycin inhibitor resistance in hepatocellular carcinoma after liver transplantation. In American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 22, 2323-2336. doi:10.1111/ajt.17124. https://pubmed.ncbi.nlm.nih.gov/35729702/
5. Huang, Xiaoyan, Wu, Zaixin, Xiao, Chunhong, Chen, X I. . E2F7/RAD51AP1 Axis Inhibits Endometrial Cancer Sensitivity to 5-FU via the Fatty Acid Metabolic Pathway. In Anticancer research, 43, 4905-4914. doi:10.21873/anticanres.16688. https://pubmed.ncbi.nlm.nih.gov/37909953/
6. Zheng, Zi-Qi, Huang, Zhuo-Hui, Liang, Ye-Lin, Tang, Ling-Long, Wei, Denghui. 2023. VIRMA promotes nasopharyngeal carcinoma, tumorigenesis, and metastasis by upregulation of E2F7 in an m6A-dependent manner. In The Journal of biological chemistry, 299, 104677. doi:10.1016/j.jbc.2023.104677. https://pubmed.ncbi.nlm.nih.gov/37028765/
7. Lin, Shengcheng, Yu, Xiangyang, Yan, Haojie, Xie, Ahuan, Yu, Zhentao. . E2F7 serves as a potential prognostic biomarker for lung adenocarcinoma. In Medicine, 103, e34342. doi:10.1097/MD.0000000000034342. https://pubmed.ncbi.nlm.nih.gov/38241554/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen